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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- PA5-89915 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- HIGD1A Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Immunogen sequence: MSTDTGVSLP SYEEDQGSKL IRKAKEAPFV PVGIAGFAAI VAYGLYKLKS RGNTKMSIHL IHMRVAAQGF VVGAMTVGMG YSMYREFWAK PKP; Positive Samples: HeLa, HepG2, 293T, HepG2, K-562; Cellular Location: Mitochondrion inner membrane, Mitochondrion membrane, Multi-pass membrane protein
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.84 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of extracts of various cell lines, using HIGD1A Polyclonal antibody (Product # PA5-89915) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Exposure time: 1s.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of HIGD1A in extracts of various cell lines using HIGD1A Polyclonal Antibody (Product # PA5-89915) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of HIGD1A was performed in U-2 OS cells using HIGD1A Polyclonal Antibody (Product # PA5-89915).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of HIGD1A in paraffin-embedded human colon carcinoma using HIGD1A Polyclonal Antibody (Product # PA5-89915) at a dilution of 1:200.
