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LearnAM06704SU-N
antibody from OriGene
Targeting: SKP1
EMC19, MGC34403, OCP-II, OCP2, p19A, SKP1A, TCEB1L
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- AM06704SU-N - Provider product page
- Provider
- OriGene
- Product name
- SKP1 mouse monoclonal antibody, clone 4E11, Ascites
- Antibody type
- Monoclonal
- Description
- SKP1 mouse monoclonal antibody, clone 4E11, Ascites
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- SKP1
- Isotype
- IgG
- Antibody clone number
- 4E11
- Vial size
- 100 µl
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis using SKP1 mAb against human SKP1 (AA: 1-160) recombinant protein. (Expected MW is 43.5 kDa)
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis using SKP1 mouse mAb against Hela (1), NIH/3T3 (2), A431 (3), RAJI (4), PC-12 (5), Cos7 (6), MCF-7 (7) and HepG2 (8) cell lysate.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Red: Control Antigen (100ng) Purple: Antigen (10ng) Green: Antigen (50ng) Blue: Antigen (100ng)
- Validation comment
- ELISA
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of Hela cells using SKP1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using SKP1 mouse mAb with DAB staining.
- Validation comment
- IHC
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Flow cytometric analysis of NIH/3T3 cells using SKP1 mouse mAb (green) and negative control (red).
- Validation comment
- FC
