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LearnMA5-15928
antibody from Invitrogen Antibodies
Targeting: SKP1
EMC19, MGC34403, OCP-II, OCP2, p19A, SKP1A, TCEB1L
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [5]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- MA5-15928 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SKP1 Monoclonal Antibody (4E11)
- Antibody type
- Monoclonal
- Antigen
- Purifed from natural sources
- Description
- MA5-15928 targets SKP1 in FACS, IF, IHC, and WB applications and shows reactivity with Human samples.
- Antibody clone number
- 4E11
- Concentration
- Conc. Not Determined
Submitted references Differential sensitivity of acute myeloid leukemia cells to daunorubicin depends on P2X7A versus P2X7B receptor expression.
Cand1-Mediated Adaptive Exchange Mechanism Enables Variation in F-Box Protein Expression.
Pegoraro A, Orioli E, De Marchi E, Salvestrini V, Milani A, Di Virgilio F, Curti A, Adinolfi E
Cell death & disease 2020 Oct 18;11(10):876
Cell death & disease 2020 Oct 18;11(10):876
Cand1-Mediated Adaptive Exchange Mechanism Enables Variation in F-Box Protein Expression.
Liu X, Reitsma JM, Mamrosh JL, Zhang Y, Straube R, Deshaies RJ
Molecular cell 2018 Mar 1;69(5):773-786.e6
Molecular cell 2018 Mar 1;69(5):773-786.e6
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of SKP1 using a SKP1 monoclonal antibody (Product # MA5-15928) against a human SKP1 (AA: 1-160) recombinant protein.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Knockdown of PAK2 was achieved by transfecting U-87 MG cells with SKP1 specific siRNAs (Silencer® select Product # s12890, s12891). Western blot analysis (Fig. a) was performed using whole cell extracts from the SKP1 knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blots were probed with SKP1 Monoclonal Antibody (Product # MA5-15928, 1:1000 dilution) and Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.25 µg/mL, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to SKP1.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed whole cell extracts (30 µg lysate) of A-431 (Lane 1), U-87 MG (Lane 2), COS-7 (Lane 3), MCF-7 (Lane 4), Hep G2 (Lane 5) and COLO 205 (Lane 6). The blot was probed with Anti-SKP1 Monoclonal Antibody (Product # MA5-15928, 1:1000 dilution) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.25 µg/mL, 1:4000 dilution). A 19 kDa band corresponding to SKP1 was observed across the cell lines tested.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of SKP1 using a SKP1 monoclonal antibody (Product # MA5-15928) against a human SKP1 (AA: 1-160) recombinant protein.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of SKP1 using SKP1 monoclonal antibody (Product # MA5-15928) in HeLa (1), NIH/3T3 (2), A431 (3), RAJI (4), PC-12 (5), COS-7 (6), MCF-7 (7) and HepG2 (8) cell lysate.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of SKP1 was performed using 70% confluent log phase MCF-7 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with SKP1 Mouse Monoclonal Antibody (4E11) (Product # MA5-15928) at 1:100 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic and nuclear localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of HeLa cells using SKP1 monoclonal antibody (Product # MA5-15928) (Green). Blue: DRAQ5 fluorescent DNA dye. Red: actin filaments have been labeled with phalloidin.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using SKP1 monoclonal antibody (Product # MA5-15928) followed with DAB staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometric analysis of NIH/3T3 cells using SKP1 monoclonal antibody (Product # MA5-15928) (green) and negative control (red).
