H00000212-M01

antibody from Abnova Corporation

Targeting: ALAS2 ASB

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Antibody Data

Product number

H00000212-M01

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00000212-M01, RRID:AB_529954

Product name

ALAS2 monoclonal antibody (M01), clone 6C1

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a partial recombinant ALAS2.

Antigen sequence

MVTAAMLLQCCPVLARGPTSLLGKVVKTHQFLFGI
GRCPILATQGPNCSQIHLKATKAGGDSPSWAKGHC
PFMLSELQDGKSKIVQKAAPEVQEDVKAFK


Isotype

IgG

Antibody clone number

6C1

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

References

Submitted references

Emodin can induce K562 cells to erythroid differentiation and improve the expression of globin genes.

Ma YN, Chen MT, Wu ZK, Zhao HL, Yu HC, Yu J, Zhang JW
Molecular and cellular biochemistry 2013 Oct;382(1-2):127-36

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Hypoxic induction of human erythroid-specific δ-aminolevulinate synthase mediated by hypoxia-inducible factor 1.

Zhang FL, Shen GM, Liu XL, Wang F, Zhao HL, Yu J, Zhang JW
Biochemistry 2011 Feb 22;50(7):1194-202

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

ALAS2 monoclonal antibody (M01), clone 6C1. Western Blot analysis of ALAS2 expression in Raw 264.7.

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of ALAS2 expression in transfected 293T cell line by ALAS2 monoclonal antibody (M01), clone 6C1.Lane 1: ALAS2 transfected lysate(64 KDa).Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged ALAS2 is approximately 0.1ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol