Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Other assay [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-96460 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- RPS10 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: MLMPKKNRIA IYELLFKEGV MVAKKDVHMP KHPELADKNV PNLHVMKAMQ SLKSRGYVKE QFAWRHFYWY LTNEGIQYLR DYLHLPPEIV PATLRRSRPE TGRPRPKGLE GERPARLTRG EADRDTYRRS AVPPGADKKA EAGAGSATEF QFRGGFGRGR GQPPQ; Positive Samples: MCF7, HL-60, A-549, HT-29, NCI-H460, Mouse spleen, Mouse heart, Mouse liver, Rat liver; Cellular Location: Cytoplasm, Nucleus, nucleolus
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 2.7 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of RPS10 in extracts of various cell lines using RPS10 Polyclonal Antibody (Product # PA5-96460) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of RPS10 was performed in MCF7 cells using RPS10 Polyclonal Antibody (Product # PA5-96460).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of RPS10 in paraffin-embedded rat brain using RPS10 Polyclonal Antibody (Product # PA5-96460) at a dilution of 1:100.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation analysis of RPS10 was performed in 200 µg extracts of MCF-7 cells using RPS10 Polyclonal Antibody (Product # PA5-96460). Western blot was performed from the immunoprecipitate using RPS10 Polyclonal Antibody at a dilution of 1:1000.