LS-B14192

antibody from LSBio

Targeting: SNRPC U1-C, Yhc1

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

LS-B14192

Provider

LSBio

Product name

IHC-plus™ SNRPC / U1C Antibody (aa109-159) LS-B14192

Antibody type

Polyclonal

Description

Affinity purified

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Storage

Store at 2-8°C for up to one year.

Western blot

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Detection of Human U1C by Western Blot. Samples: Whole cell lysate from HeLa (15 and 50 ug), and 293T (50 ug) cells. Antibodies: Affinity purified rabbit anti-U1C antibody used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Detection of Mouse U1C by Western Blot. Samples: Whole cell lysate (50 ug) from Renca and TCMK-1 cells. Antibodies: Affinity purified rabbit anti-U1C antibody used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Detection of Human U1C by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-U1C antibody used for IP at 6 ug/mg lysate. For blotting immunoprecipitated U1C, was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Detection of human Exportin-T by western blot. Samples: Whole cell lysate (50 µg) from HEK293T, HeLa, and Jurkat cells. Antibodies: Affinity purified rabbit anti-Exportin-T antibody used for WB at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.