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antibody from Invitrogen Antibodies
Targeting: LRP5
BMND1, EVR1, EVR4, HBM, LR3, LRP7, OPPG, OPS, OPTA1, VBCH2
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [6]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Other assay [6]
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- Product number
- 36-5400 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- LRP5 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 0.25 mg/mL
- Storage
- -20°C
Submitted references Circular RNA circStag1 promotes bone regeneration by interacting with HuR.
Lrp4 expression by adipocytes and osteoblasts differentially impacts sclerostin's endocrine effects on body composition and glucose metabolism.
Recombinant R-spondin2 and Wnt3a up- and down-regulate novel target genes in C57MG mouse mammary epithelial cells.
Peptide-based mediated disruption of N-cadherin-LRP5/6 interaction promotes Wnt signaling and bone formation.
Wnt signaling mediates pathological vascular growth in proliferative retinopathy.
Wnt-3a and Dickkopf-1 stimulate neurite outgrowth in Ewing tumor cells via a Frizzled3- and c-Jun N-terminal kinase-dependent mechanism.
Chen G, Long C, Wang S, Wang Z, Chen X, Tang W, He X, Bao Z, Tan B, Zhao J, Xie Y, Li Z, Yang D, Xiao G, Peng S
Bone research 2022 Mar 31;10(1):32
Bone research 2022 Mar 31;10(1):32
Lrp4 expression by adipocytes and osteoblasts differentially impacts sclerostin's endocrine effects on body composition and glucose metabolism.
Kim SP, Da H, Li Z, Kushwaha P, Beil C, Mei L, Xiong WC, Wolfgang MJ, Clemens TL, Riddle RC
The Journal of biological chemistry 2019 Apr 26;294(17):6899-6911
The Journal of biological chemistry 2019 Apr 26;294(17):6899-6911
Recombinant R-spondin2 and Wnt3a up- and down-regulate novel target genes in C57MG mouse mammary epithelial cells.
Baljinnyam B, Klauzinska M, Saffo S, Callahan R, Rubin JS
PloS one 2012;7(1):e29455
PloS one 2012;7(1):e29455
Peptide-based mediated disruption of N-cadherin-LRP5/6 interaction promotes Wnt signaling and bone formation.
Haÿ E, Buczkowski T, Marty C, Da Nascimento S, Sonnet P, Marie PJ
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research 2012 Sep;27(9):1852-63
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research 2012 Sep;27(9):1852-63
Wnt signaling mediates pathological vascular growth in proliferative retinopathy.
Chen J, Stahl A, Krah NM, Seaward MR, Dennison RJ, Sapieha P, Hua J, Hatton CJ, Juan AM, Aderman CM, Willett KL, Guerin KI, Mammoto A, Campbell M, Smith LE
Circulation 2011 Oct 25;124(17):1871-81
Circulation 2011 Oct 25;124(17):1871-81
Wnt-3a and Dickkopf-1 stimulate neurite outgrowth in Ewing tumor cells via a Frizzled3- and c-Jun N-terminal kinase-dependent mechanism.
Endo Y, Beauchamp E, Woods D, Taylor WG, Toretsky JA, Uren A, Rubin JS
Molecular and cellular biology 2008 Apr;28(7):2368-79
Molecular and cellular biology 2008 Apr;28(7):2368-79
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of (A) MCF-7 and (B) T47D cell lysates using Rb anti-LRP5 (Product # 36-5400).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of LRP5-transfected 293T cells using Rb anti-LRP5 (Product # 36-5400). Image courtesy of Heekyung Chung, PhD, and Anthony Brown, PhD, Weill Medical College of Cornell University, NY.
Supportive validation
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- Invitrogen Antibodies (provider)
- Main image
- Experimental details
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- Invitrogen Antibodies (provider)
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- Invitrogen Antibodies (provider)
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- Invitrogen Antibodies (provider)
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- Submitted by
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- Experimental details
- Figure 3 Time-course of Rspo2- and Wnt3a-dependent LRP5/6 phosphorylation and beta-catenin stabilization. C57MG cells were treated with BSA, Rspo2 (100 ng/ml), Wnt3a (100 ng/ml) alone or in combination for the indicated periods. Cell lysates (30 ug protein/lane) were immunoblotted for phosphorylated LRP5/6, total LRP5, total LRP6 and Hsp70, the latter serving as a loading control. Soluble beta-catenin was measured by immunoblotting after pull-down with GST-E-cadherin.
