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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- PAB2603 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB2603, RRID:AB_1575013
- Product name
- FZD1 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of FZD1.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references A cryptic frizzled module in cell surface collagen 18 inhibits Wnt/beta-catenin signaling.
Genomic profile of matrix and vasculature remodeling in TGF-alpha induced pulmonary fibrosis.
Bone morphogenetic protein-2 modulates Wnt and frizzled expression and enhances the canonical pathway of Wnt signaling in normal keratinocytes.
Quélard D, Lavergne E, Hendaoui I, Elamaa H, Tiirola U, Heljasvaara R, Pihlajaniemi T, Clément B, Musso O
PloS one 2008 Apr 2;3(4):e1878
PloS one 2008 Apr 2;3(4):e1878
Genomic profile of matrix and vasculature remodeling in TGF-alpha induced pulmonary fibrosis.
Hardie WD, Korfhagen TR, Sartor MA, Prestridge A, Medvedovic M, Le Cras TD, Ikegami M, Wesselkamper SC, Davidson C, Dietsch M, Nichols W, Whitsett JA, Leikauf GD
American journal of respiratory cell and molecular biology 2007 Sep;37(3):309-21
American journal of respiratory cell and molecular biology 2007 Sep;37(3):309-21
Bone morphogenetic protein-2 modulates Wnt and frizzled expression and enhances the canonical pathway of Wnt signaling in normal keratinocytes.
Yang L, Yamasaki K, Shirakata Y, Dai X, Tokumaru S, Yahata Y, Tohyama M, Hanakawa Y, Sayama K, Hashimoto K
Journal of dermatological science 2006 May;42(2):111-9
Journal of dermatological science 2006 May;42(2):111-9
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of FZD1 polyclonal antibody (Cat # PAB2603) in HeLa cell line lysates (35 ug/lane).FZD1 (arrow) was detected using the purified polyclonal antibody.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of FZD1 polyclonal antibody (Cat # PAB2603) in HeLa cells. 0.025 mg/mL primary antibody was followed by Alexa-Fluor-546-conjugated donkey anti-rabbit lgG (H+L). Alexa-Fluor-546 emits orange fluorescence. Blue counterstaining is DAPI.
- Validation comment
- Immunofluorescence
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human prostate carcinoma tissue reacted with FZD1 polyclonal antibody (Cat # PAB2603) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Flow cytometric analysis of NCI-H292 cells using FZD1 polyclonal antibody (Cat # PAB2603)(bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
- Validation comment
- Flow Cytometry
