PAB4290

antibody from Abnova Corporation

Targeting: METAP2 MAP2, MNPEP, p67

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB4290

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB4290, RRID:AB_1577222

Product name

METAP2 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against synthetic peptide of METAP2.

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

References

Submitted references

Physiologically relevant metal cofactor for methionine aminopeptidase-2 is manganese.

Wang J, Sheppard GS, Lou P, Kawai M, Park C, Egan DA, Schneider A, Bouska J, Lesniewski R, Henkin J
Biochemistry 2003 May 6;42(17):5035-42

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Negative regulation of the protection of eIF2alpha phosphorylation activity by a unique acidic domain present at the N-terminus of p67.

Datta R, Tammali R, Datta B
Experimental cell research 2003 Feb 15;283(2):237-46

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Methionine aminopeptidase 2 is a new target for the metastasis-associated protein, S100A4.

Endo H, Takenaga K, Kanno T, Satoh H, Mori S
The Journal of biological chemistry 2002 Jul 19;277(29):26396-402

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of 293 cell lysate (35 ug/lane) with METAP2 polyclonal antibody (Cat # PAB4290).

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Formalin-fixed and paraffin-embedded human hepatocellular carcinoma reacted with METAP2 polyclonal antibody (Cat # PAB4290) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining . This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated .

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)