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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- PAB2409 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB2409, RRID:AB_1581629
- Product name
- UBB polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of UBB.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references The carboxyl extensions of two rat ubiquitin fusion proteins are ribosomal proteins S27a and L40.
Differential feeding-related regulation of ubiquitin and calbindin9kDa in rat duodenum.
Structure of tetraubiquitin shows how multiubiquitin chains can be formed.
Chan YL, Suzuki K, Wool IG
Biochemical and biophysical research communications 1995 Oct 13;215(2):682-90
Biochemical and biophysical research communications 1995 Oct 13;215(2):682-90
Differential feeding-related regulation of ubiquitin and calbindin9kDa in rat duodenum.
Hubbard MJ, Carne A
Biochimica et biophysica acta 1994 Jul 6;1200(2):191-6
Biochimica et biophysica acta 1994 Jul 6;1200(2):191-6
Structure of tetraubiquitin shows how multiubiquitin chains can be formed.
Cook WJ, Jeffrey LC, Kasperek E, Pickart CM
Journal of molecular biology 1994 Feb 18;236(2):601-9
Journal of molecular biology 1994 Feb 18;236(2):601-9
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of UBB polyclonal antibody (Cat # PAB2409) in HeLa cell lysate. UBB (Arrow) was detected using purified polyclonal antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- (TOP) Formalin-fixed and paraffin-embedded human hepatocellular carcinoma reacted with UBB polyclonal antibody (Cat # PAB2409) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. (BUTTOM) Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with the UBB polyclonal antibody (Cat # PAB2409) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. HC = hepatocarcinoma.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
