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- Antibody Data
- Antigen structure
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- Validations
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- Product number
- PA5-60783 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ATP5L Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: AQFVRNLVEK TPALVNAAVT YSKPRLATFW YYAKVELVPP TPAEIPRAIQ SLKKIVNSAQ TGSFK
- Concentration
- 0.3 mg/mL
Submitted references Comparative Proteomic Profiling Identifies Reciprocal Expression of Mitochondrial Proteins Between White and Gray Matter Lesions From Multiple Sclerosis Brains.
Rai NK, Singh V, Li L, Willard B, Tripathi A, Dutta R
Frontiers in neurology 2021;12:779003
Frontiers in neurology 2021;12:779003
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Supportive validation
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- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ATP5L in Lane 1: Marker (kDa) 250, 130, 95, 72, 55, 36, 28, 17, 10; Lane 2: Human cell line RT-4; Lane 3: Human cell line U-251MG sp. Samples were probed using an ATP5L Polyclonal Antibody (Product # PA5-60783).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of ATP5L in human cell line U-2 OS shows positivity in mitochondria. Samples were probed using an ATP5L Polyclonal Antibody (Product # PA5-60783).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of ATP5L in human stomach tissue., upper shows strong cytoplasmic positivity in glandular cells. Samples were probed using an ATP5L Polyclonal Antibody (Product # PA5-60783).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 5 Cortical GML proteome profiling. (A) Volcano plot displaying the distribution of all proteins ( n = 1182) with relative protein abundance (Log2 fold change) plotted against significance level (-Log 10 P -Value). Significantly upregulated (red circle) as well as downregulated (blue circle) proteins in GMLs are also indicated. (B) Cellular identity of the significantly dysregulated GML proteins (upregulated and downregulated). (C) Venn diagram comparison of DEPs ( p < 0.05) between WMLs and GMLs. (D) IPA canonical pathways, activated (red) and inhibited (blue), associated with significantly dysregulated proteins in GMLs. (E) LFQ-based heat map of mitochondria-specific downregulated proteins in GMLs. Proteins that are common and have opposite expressions in WML proteomic data are highlighted in red. (F) PPI network analysis of downregulated proteins showing enrichment of mitochondria-related pathways. (G) MCODE analysis revealed a highly interconnected sub-network associated with oxidative phosphorylation. (H-L) PLP- stained image of (H) sub-pial lesion. IHC images showing reduced expression of NDUFA5 (I,J) and ATP5L (K,L) in demyelinated regions (red box) compared to NAGM (blue box). Scale bar- 500mum (H) and 100mum (I-L) .
