Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunoelectron microscopy [1]
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Validation data
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- Product number
- 300446 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#300446, RRID:AB_2563236
- Product name
- Alexa Fluor® 594 anti-human CD3
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- CD3ε is a 20 kD chain of the CD3/T-cell receptor (TCR) complex which is composed of two CD3ε, one CD3γ, one CD3δ, one CD3ζ (CD247), and a T-cell receptor (α/β or γ/δ) heterodimer. It is found on all mature T lymphocytes, NK-T cells, and some thymocytes. CD3, also known as T3, is a member of the immunoglobulin superfamily that plays a role in antigen recognition, signal transduction, and T cell activation.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Yellow dye
- Antibody clone number
- UCHT1
- Vial size
- 100 µg
- Concentration
- 0.5 mg/ml
- Storage
- 2°C-8°C
- Handling
- Ambient RT
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Supportive validation
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- BioLegend (provider)
- Main image
- Experimental details
- Human peripheral blood mononuclear cells were fixed with 2% paraformaldehyde (PFA), and then stained with 10 µg/ml CD14 (clone HCD14) Alexa Fluor® 647 (yellow), 10 µg/ml CD19 (clone HIB19) Alexa Fluor® 488 (green), and 10 µg/ml CD3 (clone UCHT1) Alexa Fluor® 594 (red) for 30 minutes at room temperature. Nuclei were counterstained with DAPI (blue). The image was captured with a 40X objective.
- Conjugate
- Yellow dye