DCABH-231

antibody from Creative Diagnostics

Targeting: CD3D T3D

Western blot (Recommended by provider)

Immunocytochemistry (Recommended by provider)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Recommended by provider)

Flow cytometry (Recommended by provider)

Antibody Data

Product number

DCABH-231

Provider

Creative Diagnostics

Proper citation

Creative Diagnostics Cat#DCABH-231, RRID:AB_2474296

Product name

Rabbit Anti-Human CD3D monoclonal antibody, clone KK19-08

Antibody type

Monoclonal

Description

The T cell antigen receptor (TCR) recognizes foreign antigens and translates such recognition events into intracellular signals that elicit a change in the cell from a dormant to an activated state. Much of this signaling process can be attributed to a multisubunit complex of proteins that associates directly with the TCR. This complex has been designated CD3 (cluster of differentiation 3). It is composed of five invariant polypeptide chains that associate to form three dimers: a heterodimer of gamma and epsilon chains (Îۚ÷), a heterodimer of delta and epsilon chains (Ñ´š÷) and a homodimer of two zeta chains (´Î´Î) or a heterodimer of zeta and eta chains (´Îh). The ´Î and h chains are encoded by the same gene but differ in their carboxyl-terminal ends due to an alternative splicing event. The ÎÛ, š÷ and Ñ´ chains each contain a single copy of a conserved immunoreceptor tyrosine-based activation motif (ITAM). In contrast, the ´Î chain contains three consecutive copies of the same motif. Phosphorylated ITAMs act as docking sites for protein kinases such as ZAP-70 and Syk and are also capable of regulating their kinase activity. The crystal structure of the ZAP-70 SH2 domains bound to the ´Î chain ITAMs has been solved.

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Antibody clone number

KK19-08

Vial size

100 _l

Storage

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.