60011AD

antibody from STEMCELL Technologies Inc

Targeting: CD3D T3D

Immunocytochemistry (Recommended by provider)

Flow cytometry (Recommended by provider)

Antibody Data

Product number

60011AD

Provider

STEMCELL Technologies Inc

Product name

Anti-Human CD3 Antibody, Clone UCHT1, Alexa Fluor® 488

Antibody type

Monoclonal

Description

The UCHT1 antibody reacts with the ~20 kDa CD3? subunit of the human T cell receptor (TCR)/CD3 complex, which is expressed on the surface of ~95% of mature T cells and NKT cells, and variably on thymocytes. A majority of T cell neoplasms also express CD3. The CD3 complex, which is assembled from combinations of CD3?, ?, ?, ?, and ? subunits, associates non-covalently with the TCR and is involved in transducing antigen recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR. Activation of T cells by the TCR involves the cytoplasmic tails of the CD3 subunits, which are structurally related type 1 transmembrane proteins and members of the immunoglobulin superfamily. Mutations in the CD3 subunits have been associated with various immunodeficiency disorders including severe combined immunodeficiency (SCID). This antibody clone has been verified for purity assessments of cells isolated with EasySep™ kits, including EasySep™ Direct Human T Cell Isolation Kit (Catalog #19661), EasySep™ Human CD3 Positive Selection Kit II (Catalog #17851; partial blocking may be observed), EasySep™ HLA Whole Blood T Cell Enrichment Kit (Catalog #19951HLA) and EasySep™ HLA Whole Blood CD2 Positive Selection Kit (Catalog #18687HLA).

Reactivity

Human

Isotype

IgG

Vial size

100 Tests

Storage

2 - 8°C