16669-1-AP

antibody from Invitrogen Antibodies

Targeting: CD3D T3D

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Recommended by provider)

Flow cytometry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

16669-1-AP

Provider

Invitrogen Antibodies

Product name

CD3 delta Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: FKIPIEELE DRVFVNCNTS ITWVEGTVGT LLSDITRLDL GKRILDPRGI YRCNGTDIYK DKESTVQVHY RMCQSCVELD PATVAGIIVT DVIATLLLAL GVFCFAGHET GRLSGAADTQ ALLRNDQVYQ PLRDRDDAQY SHLGGNWARN K (22-171 aa encoded by BC070321)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.13 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Mouse thymus tissue were subjected to SDS PAGE followed by western blot with 16669-1-AP (CD3 delta antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Rat thymus tissue were subjected to SDS PAGE followed by western blot with 16669-1-AP (CD3 delta antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Jurkat cells were subjected to SDS PAGE followed by western blot with 16669-1-AP (CD3 delta antibody) at dilution of 1:1000 incubated at 4 degree celsius over night.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

1X10^6 Jurkat cells were stained with 0.5ug CD3 delta antibody (16669-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L) with dilution 1:1000.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-CD3 delta (IP:16669-1-AP, 4ug; Detection:16669-1-AP 1:800) with Jurkat cells lysate 2800ug.