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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [2]
- Immunocytochemistry [1]
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Validation data
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- Product number
- H00001009-M05 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00001009-M05, RRID:AB_11188261
- Product name
- CDH11 monoclonal antibody (M05), clone 3C8
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a partial recombinant CDH11.
- Antigen sequence
GWVWNQFFVIEEYTGPDPVLVGRLHSDIDSGDGNI
KYILSGEGAGTIFVIDDKSGNIHATKTLDREERAQ
YTLMAQAVDRDTNRPLEPPSEFIVKVQDINDNPPE
FLHETYHANVPERSNVGTSVIQVTASDADDPTYGN
SAKLVYSILEGQPYFSVEAQTGIIRTALPNMDREA
KEEYHVVIQAKDMGGHMGGLSGTTKVMITLTDVND
NPPKFPQSVYQMSVSEAAVPGEEVGRVKAKDPDIG
ENGLVTYNIVDGDGMESFEITTDYETQEGVIKLKK
PVDFETKRAYSLKVEAANVHIDPKFISNGPFKDTV
TVKIAVEDADEPPMFLAPSYIHEVQENAAAGTVVG
RVHAKDPDAANSPIRYSIDRHTDLDRFFTINPEDG
FIKTTKPLDREETAWLNITVFAAEIHNRHQEAKVP
VAIRVLDVNDNAPKFAAPYEGFICESDQTKPLSNQ
PIVTISADDKDDTANGPRFIFSLPPEIIHNPNFTV
RDNRDNTAGVYARRGGFSRQKQDLYLLPIVISDGG
IPPMSSTNTLTIKVCGCDVNGALLSCNAEAYILN- Isotype
- IgG
- Antibody clone number
- 3C8
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- CDH11 monoclonal antibody (M05), clone 3C8. Western Blot analysis of CDH11 expression in MCF-7.
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- CDH11 monoclonal antibody (M05), clone 3C8. Western Blot analysis of CDH11 expression in human kidney.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged CDH11 is 1 ng/ml as a capture antibody.
- Validation comment
- Sandwich ELISA (Recombinant protein)
- Protocol
- Protocol
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged CDH11 is 3 ng/ml as a capture antibody.
- Validation comment
- Sandwich ELISA (Recombinant protein)
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- PC3-MM2 cells were stained with CDH11-FITC labeled monoclonal antibody (Green). The cell nucleus were counterstained with DAPI (Blue).
- Validation comment
- Immunofluorescence (Circulating Tumor Cell)
