NBP2-20324
antibody from Novus Biologicals
Targeting: SRSF1
ASF, MGC5228, SF2, SF2p33, SFRS1, SRp30a
Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [4]
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Validation data
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- Product number
- NBP2-20324 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Polyclonal SF2 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Mitogen-activated protein kinase-interacting kinase regulates mTOR/AKT signaling and controls the serine/arginine-rich protein kinase-responsive type 1 internal ribosome entry site-mediated translation and viral oncolysis.
Induction of viral, 7-methyl-guanosine cap-independent translation and oncolysis by mitogen-activated protein kinase-interacting kinase-mediated effects on the serine/arginine-rich protein kinase.
Brown MC, Dobrikov MI, Gromeier M
Journal of virology 2014 Nov;88(22):13149-60
Journal of virology 2014 Nov;88(22):13149-60
Induction of viral, 7-methyl-guanosine cap-independent translation and oncolysis by mitogen-activated protein kinase-interacting kinase-mediated effects on the serine/arginine-rich protein kinase.
Brown MC, Bryant JD, Dobrikova EY, Shveygert M, Bradrick SS, Chandramohan V, Bigner DD, Gromeier M
Journal of virology 2014 Nov;88(22):13135-48
Journal of virology 2014 Nov;88(22):13135-48
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: SF2 Antibody [NBP2-20324] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 12% SDS PAGE gel, diluted at 1:1000.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: SF2 Antibody [NBP2-20324] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with SF2 antibody diluted at 1:5000.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: SF2 Antibody [NBP2-20324] - Rat tissue extract (50 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with SF2 antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: SF2 Antibody [NBP2-20324] - Antibody detects SFRS1 protein at nucleus on BT483 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded BT483 xenograft. Antibody dilution: 1:500.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: SF2 Antibody [NBP2-20324] - Antibody detects SFRS1 protein at nucleus on colon carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded colon carcinoma. Antibody dilution: 1:500.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: SF2 Antibody [NBP2-20324] - Paraffin-embedded mouse skin. SF2 antibody dilution: 1:500.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: SF2 Antibody [NBP2-20324] - Paraffin-embedded mouse duodenum. SF2 antibody dilution: 1:500.