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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [4]
- Immunocytochemistry [1]
- Immunohistochemistry [4]
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Validation data
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- Product number
- GTX114918 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX114918, RRID:AB_10624593
- Product name
- SF2 antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate) A: A549 12% SDS PAGE GTX114918 diluted at 1:1000
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (50 ?g of whole cell lysate) A: mouse brain 12% SDS PAGE GTX114918 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Various whole cell extracts (30 ?g) were separated by 12% SDS-PAGE, and the membrane was blotted with SF2 antibody (GTX114918) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Rat tissue extract (50 ?g) was separated by 12% SDS-PAGE, and the membrane was blotted with SF2 antibody (GTX114918) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using SF2(GTX114918) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- SF2 antibody detects SFRS1 protein at nucleus on BT483 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded BT483 xenograft. SF2 antibody (GTX114918) dilution: 1:500.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- SF2 antibody detects SFRS1 protein at nucleus on human colon carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded colon carcinoma. SF2 antibody (GTX114918) dilution: 1:500.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- SF2 antibody detects SF2 protein at nucleus on mouse skin by immunohistochemical analysis. Sample: Paraffin-embedded mouse skin. SF2 antibody (GTX114918) dilution: 1:500.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- SF2 antibody detects SF2 protein at nucleus on mouse duodenum by immunohistochemical analysis. Sample: Paraffin-embedded mouse duodenum. SF2 antibody (GTX114918) dilution: 1:500.
