- PA5-30220 - Invitrogen Antibodies SRSF1 antibody

McClellan B, Gries P, Harlow B, Tiziani S, Jolly C, deGraffenried L
BMC cancer 2022 Sep 12;22(1):976

Umthong S, Lynch B, Timilsina U, Waxman B, Ivey EB, Stavrou S
mBio 2021 Mar 2;12(2)

Tiloke C, Phulukdaree A, Anand K, Gengan RM, Chuturgoon AA
Journal of cellular biochemistry 2016 Oct;117(10):2302-14

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Western blot analysis of SF2 using 30 µg of A549 lysate. Samples were loaded onto a 12% SDS-PAGE gel and probed with a SF2 polyclonal antibody (Product # PA5-30220) at a dilution of 1:1000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of SRSF1 was performed by separating 50 µg of rat tissue extract by 12% SDS-PAGE. Proteins were transferred to a membrane and probed with a SRSF1 Polyclonal Antibody (Product # PA5-30220) at a dilution of 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot using SRSF1 Polyclonal Antibody (Product # PA5-30220). Sample (50 µg of whole cell lysate). Lane A: mouse brain. 12% SDS PAGE. SRSF1 Polyclonal Antibody (Product # PA5-30220) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot analysis of SRSF1 was performed by separating 30 µg of various whole cell extracts by 12% SDS-PAGE. Proteins were transferred to a membrane and probed with a SRSF1 Polyclonal Antibody (Product # PA5-30220) at a dilution of 1:5000 and a HRP-conjugated anti-rabbit IgG secondary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Knockdown of SRSF1 was achieved by transfecting HeLa cells with SRSF1 specific siRNAs (Silencer® select Product # s12727, s12726). Western blot analysis (Fig. a) was performed using nuclear extracts from the SRSF1 knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blots were probed with SRSF1 Polyclonal Antibody (Product # PA5-30220, 1:1000 dilution) and Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/ml, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to SRSF1.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Western blot analysis was performed on modified whole cell extracts (30 µg lysate) of HeLa (Lane 1), PC-3 (Lane 2), MCF7 (Lane 3), U-2 OS (Lane 4), A549 (Lane 5), SH-SY5Y (Lane 6) and Mouse Brain (Lane 7). The blot was probed with SRSF1 Polyclonal Antibody (Product # PA5-30220, 1:1000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/ml, 1:4000 dilution). A 34 kDa band corresponding to SRSF1 was observed across the cell lines and tissue tested.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Immunofluorescent analysis of SF2 in paraformaldehyde-fixed HeLa cells using a SF2 polyclonal antibody (Product # PA5-30220) (Green) at a 1:500 dilution. Alpha-tubulin filaments were labeled with Product # PA5-29281 (Red) at a 1:2000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: SRSF1 Polyclonal Antibody detects SF2 protein at nucleus on mouse duodenum by immunohistochemical analysis. Sample: Paraffin-embedded mouse duodenum. SRSF1 Polyclonal Antibody (Product # PA5-30220) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: SRSF1 Polyclonal Antibody detects SF2 protein at nucleus on mouse skin by immunohistochemical analysis. Sample: Paraffin-embedded mouse skin. SRSF1 Polyclonal Antibody (Product # PA5-30220) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: SRSF1 Polyclonal Antibody detects SFRS1 protein at nucleus on BT483 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded BT483 xenograft. SRSF1 Polyclonal Antibody (Product # PA5-30220) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: SRSF1 Polyclonal Antibody detects SFRS1 protein at nucleus on human colon carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded colon carcinoma. SRSF1 Polyclonal Antibody (Product # PA5-30220) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: The effect of ML AuNP on protein levels in A549 cells. Western blot analysis of protein levels (A) and the relative fold change (B) in A549 lung cancer cells after exposure to ML AuNP (*** P < 0.0001, ** P < 0.001, and * P < 0.05). Apoptotic proteins were significantly increased with a simultaneous decrease in anti-apoptotic proteins. Protein bands were normalized against beta-actin.

PA5-30220