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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- PA5-32073 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- C5orf54 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: H1299, MCF-7.
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using C5orf54 Polyclonal Antibody (Product # PA5-32073). Sample (30 µg of whole cell lysate). Lane A: H1299. Lane B: MCF-7 . 7.5% SDS PAGE. C5orf54 Polyclonal Antibody (Product # PA5-32073) diluted at 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- C5orf54 Polyclonal Antibody detects C5orf54 protein at mitochondria by immunofluorescent analysis. Sample: H1299 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: C5orf54 protein stained by C5orf54 Polyclonal Antibody (Product # PA5-32073) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 µm.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded BT483 xenograft, using C5orf54 (Product # PA5-32073) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
