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Learn20825-1-AP
antibody from Invitrogen Antibodies
Targeting: SGSM3
DJ1042K10.2, RabGAP-5, RABGAP5, RUSC3, RUTBC3
Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [2]
- Immunohistochemistry [4]
- Other assay [1]
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- Product number
- 20825-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SGSM3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: RTQRRKSTI TALLFGEDDL EALKAKNIKQ TELVADLREA ILRVARHFQC TDPKNCSVEL TPDYSMESHQ RDHENYVACS RSHRRRAKAL LDFERHDDDE LGFRKNDIIT IVSQKDEHCW VGELNGLRGW FPAKFVEVLD ERSKEYSIAG DDSVTEGVTD LVRGTLCPAL KALFEHGLKK PSLLGGACHP WLFIEEAAGR EVERDFASVY SRLVLCKTFR LDEDGKVLTP EELLYRAVQS VNVTHDAVHA QMDVKLRSLI CVGLNEQVLH LWLEVLCSSL PTVEKWYQPW SFLRSPGWVQ IKCELRVLCC FAFSLSQDWE LPAKREAQQP LKEGVRDMLV KHHLFSWDVD G (400-749 aa encoded by BC008078)
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.32 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse brain tissue were subjected to SDS PAGE followed by western blot with 20825-1-AP (SGSM3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fetal human brain tissue were subjected to SDS PAGE followed by western blot with 20825-1-AP ( SGSM3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse testis tissue were subjected to SDS PAGE followed by western blot with 20825-1-AP (SGSM3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of ( -20°C Ethanol ) fixed HeLa cells using 20825-1-AP (SGSM3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Hela cells, using SGSM3 antibody 20825-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human spleen using 20825-1-AP (SGSM3 antibody) at dilution of 1:100 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human spleen using 20825-1-AP (SGSM3 antibody) at dilution of 1:100 (under 40x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human heart tissue slide using 20825-1-AP ( SGSM3 Antibody) at dilution of 1:200 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human heart tissue slide using 20825-1-AP ( SGSM3 Antibody) at dilution of 1:200 (under 40x lens).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-SGSM3 (IP:20825-1-AP, 4ug; Detection:20825-1-AP 1:600) with mouse brain tissue lysate 3440ug.
