Explore
Validate
Learn
Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [3]
- Flow cytometry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- NB100-57840 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-57840, RRID:AB_2110797
- Product name
- Goat Polyclonal Connexin 26/GJB2 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse, Rat
- Host
- Goat
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 0.5 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
Submitted references Connexin 26 regulates epidermal barrier and wound remodeling and promotes psoriasiform response.
Djalilian AR, McGaughey D, Patel S, Seo EY, Yang C, Cheng J, Tomic M, Sinha S, Ishida-Yamamoto A, Segre JA
The Journal of clinical investigation 2006 May;116(5):1243-53
The Journal of clinical investigation 2006 May;116(5):1243-53
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Connexin 26/GJB2 Antibody [NB100-57840] - (0.01ug/ml) staining of Mouse Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Connexin 26/GJB2 Antibody [NB100-57840] - Staining of Human Colon (A), Heart (B), Prostate (C), Skin (D) and negative control Adipose (E) lysate (35 ug protein in RIPA buffer). Antibody at 1 ug/mL. Detected by chemiluminescence.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Connexin 26/GJB2 Antibody [NB100-57840] - Staining of Mouse Brain lysate with antibody at 0.01 ug/mL (A), Mouse Heart (B) and Rat Heart (C) lysate with antibody at 0.5 ug/mL (35 ug protein in RIPA buffer). Detected by chemiluminescence.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: Connexin 26/GJB2 Antibody [NB100-57840] - Flow cytometric analysis of paraformaldehyde fixed HepG2 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
