Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
Submit
Validation data
Reference
Comment
Report error
- Product number
- AM1816a - Provider product page
- Provider
- Abgent
- Proper citation
- Abgent Cat#AM1816a, RRID:AB_2059362
- Product name
- ATG12 Antibody
- Antibody type
- Monoclonal
- Antigen
- Recombinant Protein
- Description
- Purified Mouse Monoclonal Antibody (Mab)
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 400 µl
- Concentration
- 1 mg/ml
- Storage
- Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Submitted references Activation of autophagy in mesenchymal stem cells provides tumor stromal support.
Sanchez CG, Penfornis P, Oskowitz AZ, Boonjindasup AG, Cai DZ, Dhule SS, Rowan BG, Kelekar A, Krause DS, Pochampally RR
Carcinogenesis 2011 Jul;32(7):964-72
Carcinogenesis 2011 Jul;32(7):964-72
No comments: Submit comment
Supportive validation
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- Western blot analysis of anti-APG12 Monoclonal Antibody (Cat.#AM1816a) by APG12 recombinant protein. APG12(arrow) was detected using the purified Mab.
- Primary Ab dilution
- 1:200~2000
Supportive validation
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- Atg12 antibody tested in HEK cells. Fixed in PFA permeablilized with 0.2% Saponin. Blocked and antibodies in 10% goat serum. Secondary was mouse 555. The right is in full medium (FM) and the left with EBSS (no leupeptin)(Provided by Nicole McKnight & Sharon Tooze).
- Primary Ab dilution
- 1:10~50
Supportive validation
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- "Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue and lung carcinoma tissue reacted with APG12L Monoclonal Antibody (Cat.#AM1816a), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated."
- Primary Ab dilution
- 1:50~200
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- "Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with APG12L Monoclonal Antibody (Cat.#AM1816a), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated."
- Primary Ab dilution
- 1:50~200
