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ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- STJ140014 - Provider product page
- Provider
- St John's Laboratory
- Product name
- Anti-ATG12 antibody (65aa N-Term) (STJ140014)
- Antibody type
- Polyclonal
- Description
- Goat polyclonal antibody anti-Autophagy related 12 (65aa N-Term) is suitable for use in Western Blot and Immunofluorescence research applications.
- Reactivity
- Human, Mouse, Rat, Canine, Simian
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
NA- Epitope
- NA
- Isotype
- IgG
- Antibody clone number
- NA
- Vial size
- NA
- Concentration
- NA
- Storage
- For continuous use, store at 2-8 C for one-two days. For extended storage, store in-20 C freezer. Working dilution samples should be discarded if not used within 12 hours.
- Handling
- NA
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Supportive validation
- Submitted by
- St John's Laboratory (provider)
- Main image
- Experimental details
- Immunofluorescence – anti-ATG12 antibody – Autophagosome Marker in Hepa1-6 cells at 1:50 dilution; cells were fixed with methanol
- Sample type
- NA
- Validation comment
- NA
- Primary Ab dilution
- NA
- Other comments
- NA
- Secondary Ab
- NA
- Secondary Ab dilution
- NA
- Protocol
- NA
Supportive validation
- Submitted by
- St John's Laboratory (provider)
- Main image
- Experimental details
- Anti-Atg12 antibody at 1:500 dilution; 293HEK cells transfected with GFP-Atg12; lysate at 100 µg per lane; rabbit polyclonal to goat IgG (HRP) at 1:10000 dilution
- Sample type
- NA
- Validation comment
- NA
- Primary Ab dilution
- NA
- Other comments
- NA
- Secondary Ab
- NA
- Secondary Ab dilution
- NA
- Protocol
- NA
