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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
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- Product number
- PA5-88313 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- RPL38 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: MPRKIEEIKD FLLTARRKDA KSVKIKKNKD NVKFKVRCSR YLYTLVITDK EKAEKLKQSL PPGLAVKELK; Positive Samples: A-375, HeLa, MCF-7
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 2.9 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references Knockdown of the Ribosomal Protein eL38 in HEK293 Cells Changes the Translational Efficiency of Specific Genes.
Gopanenko AV, Kolobova AV, Tupikin AE, Kabilov MR, Malygin AA, Karpova GG
International journal of molecular sciences 2021 Apr 26;22(9)
International journal of molecular sciences 2021 Apr 26;22(9)
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of extracts of various cell lines, using RPL38 Polyclonal antibody (Product # PA5-88313) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Exposure time: 30s.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of RPL38 in extracts of various cell lines using RPL38 Polyclonal Antibody (Product # PA5-88313) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 1 Ribosomal protein eL38 knockdown in HEK293 cells. ( A ) Western blot analysis of the levels of eL38 and GAPDH (as a reference) in cells transfected with siRNAs specific for eL38 mRNA (1) and non-targeting siRNA (2). The diagram shows triplicate western blot data as the mean of arbitrary units (a.u.) +- SEM (** p < 0.01, calculated using Mann-Whitney test). ( B ) Sucrose gradient polysome profiles obtained by the centrifugation of the lysates of cells transfected with non-targeting siRNA (solid line) and eL38 mRNA-specific siRNAs (dashed line); the peaks of 60S and 40S subunits, 80S ribosomes and polysomes are marked. Western blot analysis of the sucrose gradient fractions for the presence of ribosomal proteins eL38 and eS4 (as a reference).
