Explore
Validate
Learn
Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [4]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
- Flow cytometry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- MA5-17100 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- INCENP Monoclonal Antibody (3D2)
- Antibody type
- Monoclonal
- Antigen
- Purifed from natural sources
- Description
- MA5-17100 targets INCENP in indirect ELISA, FACS, ICC, IHC, IF and WB applications and shows reactivity with Human samples. The MA5-17100 immunogen is purified recombinant fragment of human INCENP (amino acids: 369-583) expressed in E. Coli. MA5-17100 detects INCENP which has a predicted molecular weight of approximately 105.4kDa.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 3D2
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Unraveling mitotic protein networks by 3D multiplexed epitope drug screening.
Maier LJ, Kallenberger SM, Jechow K, Waschow M, Eils R, Conrad C
Molecular systems biology 2018 Aug 13;14(8):e8238
Molecular systems biology 2018 Aug 13;14(8):e8238
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of INCENP using a INCENP monoclonal antibody (Product # MA5-17100) against a human INCENP recombinant protein.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of INCENP using INCENP monoclonal antibody (Product # MA5-17100) in HEK293 (1) and INCENP (AA: 369-583) human IgG Fc transfected HEK293 (2) cell lysate.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of INCENP using a INCENP monoclonal antibody (Product # MA5-17100) against a human INCENP recombinant protein.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of INCENP using INCENP monoclonal antibody (Product # MA5-17100) in HEK293 (1) and INCENP (AA: 369-583) human IgG Fc transfected HEK293 (2) cell lysate.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of HepG2 cells using INCENP monoclonal antibody (Product # MA5-17100) (Green). Blue: DRAQ5 fluorescent DNA dye.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using INCENP monoclonal antibody (Product # MA5-17100) followed with DAB staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using INCENP monoclonal antibody (Product # MA5-17100) followed with DAB staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometric analysis of Jurkat cells using INCENP monoclonal antibody (Product # MA5-17100) (green) and negative control (red).
