Ab115549

antibody from Aladdin Scientific Corporation

Targeting: MLH1 COCA2, FCC2, HNPCC, HNPCC2

Western blot (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Antibody Data

Product number

Ab115549

Provider

Aladdin Scientific Corporation

Product name

Recombinant MLH1 Antibody

Antibody type

Recombinant

Description

Rabbit anti Human MLH1 Antibody, Recombinant (R07-5D3), could be used for WB, ICC, IF and so on.Application:WB: 1/500-1/1000ICC/IF: 1/50-1/200 Protein FunctionHeterodimerizes with PMS2 to form MutL alpha, a component of the post-replicative DNA mismatch repair system (MMR). DNA repair is initiated by MutS alpha (MSH2-MSH6) or MutS beta (MSH2-MSH6) binding to a dsDNA mismatch, then MutL alpha is recruited to the heteroduplex. Assembly of the MutL-MutS-heteroduplex ternary complex in presence of RFC and PCNA is sufficient to activate endonuclease activity of PMS2. It introduces single-strand breaks near the mismatch and thus generates new entry points for the exonuclease EXO1 to degrade the strand containing the mismatch. DNA methylation would prevent cleavage and therefore assure that only the newly mutated DNA strand is going to be corrected. MutL alpha (MLH1-PMS2) interacts physically with the clamp loader subunits of DNA polymerase III, suggesting that it may play a role to recruit the DNA polymerase III to the site of the MMR. Also implicated in DNA damage signaling, a process which induces cell cycle arrest and can lead to apoptosis in case of major DNA damages. Heterodimerizes with MLH3 to form MutL gamma which plays a role in meiosis.

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Antigen sequence

AA 350-756


Antibody clone number

R07-5D3

Vial size

100μl,10μl,1ml,50μl

Concentration

0,6 mg/ml

Storage

Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.