Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- 848502 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#848502, RRID:AB_2650692
- Product name
- Purified anti-Neurogranin, 70-78
- Antibody type
- Monoclonal
- Reactivity
- Human, Rat
- Host
- Mouse
- Conjugate
- Unconjugated
- Antibody clone number
- A16007A
- Vial size
- 100 µg
- Concentration
- 0.5 mg/ml
- Storage
- 2°C-8°C
- Handling
- Ambient RT
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Supportive validation
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- BioLegend (provider)
- Main image
- Experimental details
- Western blot of purified anti-Neurogranin antibody (clone A16007A) and isotype-matched IgG2b control, demonstrating antibody specificity towards endogenous neurogranin protein. Lane 1: 20 µg of human brain tissue lysate. Lane 2: 20 µg of human Alzheimer's disease brain tissue lysate. Lanes 3 and 4: 5 µg of rat brain tissue lysate. Lane 5: 50 ng of human recombinant Neurogranin-GST protein. Blots were incubated with 1 µg/mL of primary antibody overnight at 4°C followed by incubation with HRP labeled goat anti-mouse secondary antibody. Enhanced chemiluminescence was used as the detection system.
- Conjugate
- Unconjugated