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ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [14]
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- Validations
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- V3738 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- ATRX Antibody / RAD54
- Antibody type
- Monoclonal
- Description
- Protein G affinity chromatography
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antibody clone number
- 39f
- Vial size
- 20 µg, 100 µg (with BSA, sodium azide), 100 µg (without BSA, sodium azide), 7 ml (prediluted for IHC)
- Concentration
- 0.2 mg/ml, 1 mg/ml (BSA/azide free format), Prediluted (IHC only format)
- Storage
- Store the ATRX antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Submitted references Stimulation of the Replication of ICP0-Null Mutant Herpes Simplex Virus 1 and pp71-Deficient Human Cytomegalovirus by Epstein-Barr Virus Tegument Protein BNRF1.
ATRX dysfunction induces replication defects in primary mouse cells.
The replication defect of ICP0-null mutant herpes simplex virus 1 can be largely complemented by the combined activities of human cytomegalovirus proteins IE1 and pp71.
Control of human adenovirus type 5 gene expression by cellular Daxx/ATRX chromatin-associated complexes.
Localization of the chromatin remodelling protein, ATRX in the adult testis.
Functional significance of mutations in the Snf2 domain of ATRX.
ATR-X syndrome protein targets tandem repeats and influences allele-specific expression in a size-dependent manner.
Regulation of ICP0-null mutant herpes simplex virus type 1 infection by ND10 components ATRX and hDaxx.
Loss of ATRX leads to chromosome cohesion and congression defects.
Human cytomegalovirus protein pp71 displaces the chromatin-associated factor ATRX from nuclear domain 10 at early stages of infection.
Attenuation of an amino-terminal premature stop codon mutation in the ATRX gene by an alternative mode of translational initiation.
Cell cycle-dependent phosphorylation of the ATRX protein correlates with changes in nuclear matrix and chromatin association.
Mutations in ATRX, encoding a SWI/SNF-like protein, cause diverse changes in the pattern of DNA methylation.
Localization of a putative transcriptional regulator (ATRX) at pericentromeric heterochromatin and the short arms of acrocentric chromosomes.
Lu Y, Orr A, Everett RD
Journal of virology 2016 Nov 1;90(21):9664-9673
Journal of virology 2016 Nov 1;90(21):9664-9673
ATRX dysfunction induces replication defects in primary mouse cells.
Clynes D, Jelinska C, Xella B, Ayyub H, Taylor S, Mitson M, Bachrati CZ, Higgs DR, Gibbons RJ
PloS one 2014;9(3):e92915
PloS one 2014;9(3):e92915
The replication defect of ICP0-null mutant herpes simplex virus 1 can be largely complemented by the combined activities of human cytomegalovirus proteins IE1 and pp71.
Everett RD, Bell AJ, Lu Y, Orr A
Journal of virology 2013 Jan;87(2):978-90
Journal of virology 2013 Jan;87(2):978-90
Control of human adenovirus type 5 gene expression by cellular Daxx/ATRX chromatin-associated complexes.
Schreiner S, Bürck C, Glass M, Groitl P, Wimmer P, Kinkley S, Mund A, Everett RD, Dobner T
Nucleic acids research 2013 Apr 1;41(6):3532-50
Nucleic acids research 2013 Apr 1;41(6):3532-50
Localization of the chromatin remodelling protein, ATRX in the adult testis.
Tang P, Argentaro A, Pask AJ, O'Donnell L, Marshall-Graves J, Familari M, Harley VR
The Journal of reproduction and development 2011 Jun;57(3):317-21
The Journal of reproduction and development 2011 Jun;57(3):317-21
Functional significance of mutations in the Snf2 domain of ATRX.
Mitson M, Kelley LA, Sternberg MJ, Higgs DR, Gibbons RJ
Human molecular genetics 2011 Jul 1;20(13):2603-10
Human molecular genetics 2011 Jul 1;20(13):2603-10
ATR-X syndrome protein targets tandem repeats and influences allele-specific expression in a size-dependent manner.
Law MJ, Lower KM, Voon HP, Hughes JR, Garrick D, Viprakasit V, Mitson M, De Gobbi M, Marra M, Morris A, Abbott A, Wilder SP, Taylor S, Santos GM, Cross J, Ayyub H, Jones S, Ragoussis J, Rhodes D, Dunham I, Higgs DR, Gibbons RJ
Cell 2010 Oct 29;143(3):367-78
Cell 2010 Oct 29;143(3):367-78
Regulation of ICP0-null mutant herpes simplex virus type 1 infection by ND10 components ATRX and hDaxx.
Lukashchuk V, Everett RD
Journal of virology 2010 Apr;84(8):4026-40
Journal of virology 2010 Apr;84(8):4026-40
Loss of ATRX leads to chromosome cohesion and congression defects.
Ritchie K, Seah C, Moulin J, Isaac C, Dick F, Bérubé NG
The Journal of cell biology 2008 Jan 28;180(2):315-24
The Journal of cell biology 2008 Jan 28;180(2):315-24
Human cytomegalovirus protein pp71 displaces the chromatin-associated factor ATRX from nuclear domain 10 at early stages of infection.
Lukashchuk V, McFarlane S, Everett RD, Preston CM
Journal of virology 2008 Dec;82(24):12543-54
Journal of virology 2008 Dec;82(24):12543-54
Attenuation of an amino-terminal premature stop codon mutation in the ATRX gene by an alternative mode of translational initiation.
Howard MT, Malik N, Anderson CB, Voskuil JL, Atkins JF, Gibbons RJ
Journal of medical genetics 2004 Dec;41(12):951-6
Journal of medical genetics 2004 Dec;41(12):951-6
Cell cycle-dependent phosphorylation of the ATRX protein correlates with changes in nuclear matrix and chromatin association.
Bérubé NG, Smeenk CA, Picketts DJ
Human molecular genetics 2000 Mar 1;9(4):539-47
Human molecular genetics 2000 Mar 1;9(4):539-47
Mutations in ATRX, encoding a SWI/SNF-like protein, cause diverse changes in the pattern of DNA methylation.
Gibbons RJ, McDowell TL, Raman S, O'Rourke DM, Garrick D, Ayyub H, Higgs DR
Nature genetics 2000 Apr;24(4):368-71
Nature genetics 2000 Apr;24(4):368-71
Localization of a putative transcriptional regulator (ATRX) at pericentromeric heterochromatin and the short arms of acrocentric chromosomes.
McDowell TL, Gibbons RJ, Sutherland H, O'Rourke DM, Bickmore WA, Pombo A, Turley H, Gatter K, Picketts DJ, Buckle VJ, Chapman L, Rhodes D, Higgs DR
Proceedings of the National Academy of Sciences of the United States of America 1999 Nov 23;96(24):13983-8
Proceedings of the National Academy of Sciences of the United States of America 1999 Nov 23;96(24):13983-8
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of FFPE human pancreas with ATRX antibody (clone 39f). Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- SDS-PAGE analysis of purified, BSA-free ATRX antibody (clone 39f) as confirmation of integrity and purity.
