- PA5-21348 - Invitrogen Antibodies ATRX antibody

Submitted references ATRX proximal protein associations boast roles beyond histone deposition.

Scott WA, Dhanji EZ, Dyakov BJA, Dreseris ES, Asa JS, Grange LJ, Mirceta M, Pearson CE, Stewart GS, Gingras AC, Campos EI
PLoS genetics 2021 Nov;17(11):e1009909

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of ATRX using Various whole cell extracts (30 µg). Samples were loaded onto a 5% SDS-PAGE gel and probed with an ATRX polyclonal antibody (Product # PA5-21348) at a dilution of 1:1000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of ATRX using 30 µg NIH-3T3 whole cell extract. Samples were loaded onto a 5% SDS-PAGE gel and probed with an ATRX polyclonal antibody (Product # PA5-21348) at a dilution of 1:1000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of ATRX using Various whole cell extracts (30 µg). Samples were loaded onto a 5% SDS-PAGE gel and probed with an ATRX polyclonal antibody (Product # PA5-21348) at a dilution of 1:1000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot analysis of ATRX was performed by separating 30 µg of various whole cell extracts by 5% SDS-PAGE. Proteins were transferred to a membrane and probed with a ATRX Polyclonal Antibody (Product # PA5-21348) at a dilution of 1:1000 and a HRP-conjugated anti-rabbit IgG secondary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: ATRX antibody detects ATRX protein by western blot analysis. Whole cell extracts (30 µg) was separated by 5% SDS-PAGE, and the membrane was blotted with ATRX antibody ATRX Polyclonal Antibody (Product # PA5-21348) diluted by 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot using ATRX Polyclonal Antibody (Product # PA5-21348). Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with ATRX Polyclonal Antibody (Product # PA5-21348) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunocytochemistry-Immunofluorescence analysis of ATRX was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: ATRX Polyclonal Antibody (Product # PA5-21348) diluted at 1:1500. Red: phalloidin, a cytoskeleton marker.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: ATRX Polyclonal Antibody detects ATRX protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ATRX stained by ATRX Polyclonal Antibody (Product # PA5-21348) diluted at 1:1,000. Red: phalloidin, a cytoskeleton marker, diluted at 1:200. Scale bar= 10 µm.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry (Paraffin) analysis of ATRX was performed in paraffin-embedded human breast carcinoma tissue using ATRX Polyclonal Antibody (Product # PA5-21348) at a dilution of 1:2000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry (Paraffin) analysis of ATRX was performed in paraffin-embedded human lung cancer tissue using ATRX Polyclonal Antibody (Product # PA5-21348) at a dilution of 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: ATRX Polyclonal Antibody detects ATRX protein at cytoplasm and nucleus by immunohistochemical analysis. Sample: Paraffin-embedded human lung cancer. ATRX stained by ATRX Polyclonal Antibody (Product # PA5-21348) diluted at 1:2,500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Rad54 antibody [C3], C-term detects Rad54 protein at nucleus on human endometrial carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human endometrial carcinoma. Rad54 antibody [C3], C-term (Product # PA5-21348) dilution: 1:500.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: 10.1371/journal.pgen.1009909.g004 Fig 4 Loss of ATRX and SLF2 enables telomere exchanges. A: DNA sequencing and western blotting confirming the disruption of ATRX expression in CRISPR-Cas9-edited HEK293 Flp-In T-REx cells inducibly expressing RAP1-BirA*. B: Relative signal overlap between immunolabeled SLF2 and telomere fluorescence in situ hybridization (IF-FISH), in ATRX-expressing or - HEK293 Flp-In cells. Cells were exposed to 10 muM pyridostatin (PDS, G4 stabilizer) for 60 min. At least 40 cells per condition were analyzed using a CellProfiler colocalization pipeline []. ATRX loss decreased SLF2 recruitment to telomeres in the absence of pyridostatin. C: Schematic of dual-colour chromosome-orientation fluorescence in situ hybridization (CO-FISH) [] using PNA probes to label C- and G-rich telomere strands (left). Examples of normal CO-FISH signals and of a chromosome end with a telomere exchange are shown (right). D: Relative amount of telomere exchanges in ATRX, SLF2, and ATRX/SLF2 KO HEK293 Flp-In cells. At least 30 mitotic spreads per condition were counted and the percent of telomeric exchanges plotted. p-values were obtained using a 2-sided Student's t-test with unequal variance.

PA5-21348

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