NBP1-32851

antibody from Novus Biologicals

Targeting: ATRX JMS, MRX52, RAD54, XH2, XNP

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP1-32851

Provider

Novus Biologicals

Proper citation

Novus Cat#NBP1-32851, RRID:AB_2061035

Product name

Rabbit Polyclonal ATRX Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

0.1 ml

Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

References

Submitted references

Dysregulation of select ATP-dependent chromatin remodeling factors in high trait anxiety.

Wille A, Amort T, Singewald N, Sartori SB, Lusser A
Behavioural brain research 2016 Sep 15;311:141-146

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Impaired Contextual Fear Extinction Learning is Associated with Aberrant Regulation of CHD-Type Chromatin Remodeling Factors.

Wille A, Maurer V, Piatti P, Whittle N, Rieder D, Singewald N, Lusser A
Frontiers in behavioral neuroscience 2015;9:313

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: ATRX Antibody [NBP1-32851] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ATRX antibody [C3], C-term diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: ATRX Antibody [NBP1-32851] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ATRX antibody [C3], C-term.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: ATRX Antibody [NBP1-32851] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ATRX antibody diluted at a dilution of 1:1000.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: ATRX Antibody [NBP1-32851] - Whole cell extracts (30 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with ATRX antibody diluted by 1:1000.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: ATRX Antibody [NBP1-32851] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ATRX antibody diluted at a dilution of 1:1000.

Immunoprecipitation

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunoprecipitation: ATRX Antibody [NBP1-32851] - Immunoprecipitation of ATRX protein from 293T whole cell extracts using 5 ug of ATRX antibody [C3], C-term (NBP1-32851). Western blot analysis was performed using ATRX antibody [C3], C-term (NBP1-32851). EasyBlot anti-Rabbit IgG was used as a secondary reagent.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: ATRX Antibody [NBP1-32851] - Human endometrial carcinoma dilution: 1:500.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: ATRX Antibody [NBP1-32851] - human breast carcinoma dilution: 1:500.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: ATRX Antibody [NBP1-32851] - Human lung carcinoma dilution: 1:500

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: ATRX Antibody [NBP1-32851] - Human breast carcinoma. ATRX stained by ATRX antibody [C3], C-term diluted at 1:2000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.