LS-C47555

antibody from LSBio

Targeting: PAX3 HUP2, WS1

Western blot (Data presented on provider website)

ELISA (Recommended by provider)

Immunohistochemistry (Enhanced validation)

Antibody Data

Product number

LS-C47555

Provider

LSBio

Product name

PAX3 Antibody (aa2-12) LS-C47555

Antibody type

Polyclonal

Description

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Reactivity

Human, Mouse, Rat, Bovine, Canine, Chicken/Avian, Hamster, Horse, Rabbit

Host

Goat

Storage

Aliquot and store at -20°C. Avoid freeze-thaw cycles.

Immunohistochemistry

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Negative Control showing staining of paraffin embedded Human Esophagus, with no primary antibody.

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

PAX3 antibody (3 ug/ml) staining of paraffin embedded Human Esophagus. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Negative Control showing staining of paraffin embedded Human Esophagus, with no primary antibody.

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

PAX3 antibody (3µg/ml) staining of paraffin embedded Human Oesophagus. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining. Data obtained using previous batch.

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

PAX3 antibody (4.5µg/ml) staining of paraffin embedded Human Esophagus. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Negative Control showing staining of paraffin embedded Human Esophagus, with no primary antibody.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

PAX3 antibody (3µg/ml) staining of paraffin embedded Human Oesophagus. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining. Data obtained using previous batch.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

PAX3 antibody (4.5µg/ml) staining of paraffin embedded Human Esophagus. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

PAX3 antibody (3 ug/ml) staining of paraffin embedded Human Esophagus. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Negative Control showing staining of paraffin embedded Human Esophagus, with no primary antibody.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

PAX3 antibody (3µg/ml) staining of paraffin embedded Human Oesophagus. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining. Data obtained using previous batch.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

PAX3 antibody (4.5µg/ml) staining of paraffin embedded Human Esophagus. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.