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ImmunocytochemistryAntibody data
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- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Flow cytometry [5]
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- Product number
- NBP2-42216 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP2-42216, RRID:AB_2801654
- Product name
- Mouse Monoclonal SLC34A1 Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified.
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1.0 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
Submitted references 1,25-Dihydroxyvitamin D Maintains Brush Border Membrane NaPi2a and Attenuates Phosphaturia in Hyp Mice.
Martins JS, Liu ES, Sneddon WB, Friedman PA, Demay MB
Endocrinology 2019 Oct 1;160(10):2204-2214
Endocrinology 2019 Oct 1;160(10):2204-2214
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: SLC34A1 Antibody (10B1.3E9) [NBP2-42216] - WB detection of SLC34A1 /NPTIIa protein in a lysate of mouse thymus using SLC34A1 clone 10B1.3E9 at its 1:500 dilution. The antibody detected a single specific band at ~80 kDa representing the glycosylated form of SLC34A1 protein.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: SLC34A1 Antibody (10B1.3E9) [NBP2-42216] - IHC analysis of a formalin-fixed and paraffin-embedded tissue section of human normal kidney using SLC34A1 antibody (clone 10B1.3E9) at 1:75 dilution. The epithelial cells of various renal ducts and tubules depicted very nice membrane-cytoplasmic SLC34A1 immunostaining while the Bowman's capsule and the nuclei of cells were largely negative for SLC34A1 protein.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: SLC34A1 Antibody (10B1.3E9) [NBP2-42216] - FLOW detection of SLC34A1 protein on HEK293 cells - After fixation and permeabilization, 2 x 10^6 cells/ml were stained using SLC34A1 antibody (clone 10B1.3E9) at 1:1000 dilution. Signal was developped using GtxMs dylight 488 secondary (blue peak). Shown with secondary control (orange peak). Data was acquired on BD FACSCalibur.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: SLC34A1 Antibody (10B1.3E9) [NBP2-42216] - An intracellular stain was performed on Hek293 cells with SLC34A1 Antibody [10B1.3E9] NBP2-42216AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: SLC34A1 Antibody (10B1.3E9) [NBP2-42216] - An intracellular stain was performed on Hek293 cells with SLC34A1 Antibody [10B1.3E9] NBP2-42216APC (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Allophycocyanin.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: SLC34A1 Antibody (10B1.3E9) [NBP2-42216] - An intracellular stain was performed on Hek293 cells with SLC34A1 Antibody [10B1.3E9] NBP2-42216PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: SLC34A1 Antibody (10B1.3E9) [NBP2-42216] - An intracellular stain was performed on Hek293 cells with SLC34A1 Antibody [10B1.3E9] NBP2-42216PCP (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to PerCP.
