AB0094-200

antibody from SICGEN

Targeting: CAV2 CAV

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

AB0094-200

Provider

SICGEN

Product name

Anti-CAV2

Antibody type

Polyclonal

Description

Goat polyclonal antibody to Catenin (cadherin-associated protein), beta 1. Beta Catenin is a 88 kDa protein and is a key downstream effector in the Wnt signalling pathway. It is involved in early embryonic development and tumorigenesis. Beta Catenin is part of a complex of proteins that constitute adherens junctions, necessary for the creation and maintenance of epithelial cell layers by regulating cell growth and adhesion between cells. This protein anchors the actin cytoskeleton and may be responsible for transmitting the contact inhibition signal that causes cells to stop dividing once the epithelial sheet is complete.

Reactivity

Human, Mouse, Rat, Bovine, Canine, Chicken/Avian, Donkey, Feline, Goat, Guinea Pig, Hamster, Horse, Porcine, Rabbit, Sheep, Simian, Other

Host

Goat

Conjugate

Unconjugated

Isotype

IgG

Vial size

600 µg

Concentration

3 mg/ml

Storage

Store at -20 C for long-term storage. Store at 2-8 C for up to one month.

Handling

The antibody solution should be gently mixed before use.

Western blot

Supportive validation

Submitted by

SICGEN (provider)

Main image

Experimental details

50 µg lysate per lane of transfected HEK293 and 100 µg of HaCat cell line

Sample type

transfected HEK293 and HaCat cell line

Primary Ab dilution

1/1,000

Conjugate

Unconjugated

Secondary Ab

Secondary Ab

Secondary Ab dilution

1/10,000

Immunocytochemistry

Supportive validation

Submitted by

SICGEN (provider)

Main image

Experimental details

Immunofluorescence – anti-CAV2 Ab at 1/100 dilution in NHI/3T3 cells; cells were fixed with methanol and permeabilized with 0.1% saponin;