PA5-81297

antibody from Invitrogen Antibodies

Targeting: PTBP1 HNRNP-I, HNRPI, pPTB, PTB, PTB-1, PTB2, PTB3, PTB4

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-81297

Provider

Invitrogen Antibodies

Product name

PTBP1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Glia-to-Neuron Conversion by CRISPR-CasRx Alleviates Symptoms of Neurological Disease in Mice.

Zhou H, Su J, Hu X, Zhou C, Li H, Chen Z, Xiao Q, Wang B, Wu W, Sun Y, Zhou Y, Tang C, Liu F, Wang L, Feng C, Liu M, Li S, Zhang Y, Xu H, Yao H, Shi L, Yang H
Cell 2020 Apr 30;181(3):590-603.e16

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using PTBP1 Polyclonal Antibody (Product # PA5-81297) at 1:500 dilution. Lane A: HepG2 Whole Cell Lysate, Lane B: Jurkat Whole Cell Lysate, Lane C: Hela Whole Cell Lysate, Lane D: A431 Whole Cell Lysate. Lysates/proteins at 30 μg per lane. Secondary antibody: Goat Anti-Rabbit IgG H&L (DyLight™ 800) at 1:10,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 57 kDa. Observed band size: 57 kDa.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence staining of PTBP1 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with PTBP1 Polyclonal Antibody (Product # PA5-81297, 1:1,000) at 4°C overnight. Then cells were stained with the Alexa Fluor®594-conjugated Goat Anti-rabbit IgG secondary antibody (red). Positive staining was localized to nucleus.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of PTBP1 in human breast carcinoma with PTBP1 Polyclonal Antibody (Product # PA5-81297, 1:2,000, formalin-fixed paraffin embedded sections).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of PTBP1 in human kidney with PTBP1 Polyclonal Antibody (Product # PA5-81297, 1:2,000, formalin-fixed paraffin embedded sections).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of PTBP1 in human placenta with PTBP1 Polyclonal Antibody (Product # PA5-81297, 1:2,000, formalin-fixed paraffin embedded sections).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of PTBP1 in mouse kidney with PTBP1 Polyclonal Antibody (Product # PA5-81297, 1:2,000 dilution, formalin-fixed paraffin embedded sections).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of PTBP1 in mouse small intestine with PTBP1 Polyclonal Antibody (Product # PA5-81297, 1:2,000 dilution, formalin-fixed paraffin embedded sections).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of PTBP1 in rat kidney with PTBP1 Polyclonal Antibody (Product # PA5-81297, 1:2,000 dilution, formalin-fixed paraffin embedded sections).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

PTBP1 Immunoprecipitation using: Lane A: 0.5 mg Jurkat Whole Cell Lysate, Lane B: 0.5 mg HepG2 Whole Cell Lysate 4 µL with PTBP1 Polyclonal Antibody (Product # PA5-81297) and 15 µL of 50 % Protein G agarose. Primary antibody: PTBP1 Polyclonal Antibody, at 1:100 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 57 kDa. Observed band size: 57 kDa.