Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- 630101 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#630101, RRID:AB_2171285
- Product name
- Purified anti-PTBP1
- Antibody type
- Monoclonal
- Antigen
- Recombinant human PTBP1 (full length)
- Description
- The 3H7 monoclonal antibody recognizes human PTBP1 also known as Polypyrimidine tract binding protein 1, Heterogeneous nuclear ribonucleoprotein I, and 57 kD RNA-binding protein. PTBP1 is a ubiquitously expressed nuclear protein with a predicted molecular weight approximately 59.6 kD. The polypyrimidine tract binding protein (PTB) is an important regulator of alternative splicing that also affects mRNA localization, stabilization, polyadenylation, and translation. PTBP1 has been shown to interact with FUS, Caspase3, PKCa, Raver1, and HnRNP-L protein. The PTB gene contains 15 exons and spans 13.5 kb. The 3H7 antibody has been shown to be useful for Western blotting.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 25 µg
- Concentration
- 0.5 mg/ml
- Storage
- The antibody solution should be stored undiluted at 4°C.
Submitted references Heat shock transcription factor Hsf1 is involved in tumor progression via regulation of hypoxia-inducible factor 1 and RNA-binding protein HuR.
Gabai VL, Meng L, Kim G, Mills TA, Benjamin IJ, Sherman MY
Molecular and cellular biology 2012 Mar;32(5):929-40
Molecular and cellular biology 2012 Mar;32(5):929-40
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Supportive validation
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- BioLegend (provider)
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- Experimental details
- Extracts from human PBMC were resolved by electrophoresis, transferred to nitrocellulose, and probed with monoclonal antibody against PTBP1 (clone 3H7). Proteins were visualized using a goat anti-mouse secondary conjugated to HRP and a chemiluminescence detection system.
- Conjugate
- Unconjugated