NB100-1310

antibody from Novus Biologicals

Targeting: PTBP1 HNRNP-I, HNRPI, pPTB, PTB, PTB-1, PTB2, PTB3, PTB4

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

NB100-1310

Provider

Novus Biologicals

Proper citation

Novus Cat#NB100-1310, RRID:AB_10001705

Product name

Goat Polyclonal PTBP1 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. This antibody is expected to recognise all 4 reported isoforms (represented by NP_002810.1

Reactivity

Human, Mouse, Rat

Host

Goat

Isotype

IgG

Vial size

0.1 mg

Concentration

0.5 mg/ml

Storage

Store at -20C. Avoid freeze-thaw cycles.

References

Submitted references

Hydrogen peroxide alters splicing of soluble guanylyl cyclase and selectively modulates expression of splicing regulators in human cancer cells.

Cote GJ, Zhu W, Thomas A, Martin E, Murad F, Sharina IG
PloS one 2012;7(7):e41099

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Dynamic antagonism between ETR-3 and PTB regulates cell type-specific alternative splicing.

Charlet-B N, Logan P, Singh G, Cooper TA
Molecular cell 2002 Mar;9(3):649-58

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Dynamic antagonism between ETR-3 and PTB regulates cell type-specific alternative splicing.

Charlet-B N, Logan P, Singh G, Cooper TA
Molecular cell 2002 Mar;9(3):649-58

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: PTBP1 Antibody [NB100-1310] - (0.01ug/ml) staining of HeLa (A), HepG2 (B), Jurkat (C) and HEK293 (D) nuclear lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: PTBP1 Antibody [NB100-1310] - (0.1-0.3ug/ml) staining of Mouse Ovary (A) and Rat Ovary (B) lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: PTBP1 Antibody [NB100-1310] - (0.03ug/ml) staining of lysates of cell line NIH3T3 (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: PTBP1 Antibody [NB100-1310] - (0.1ug/ml) staining of Human Ovary lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: PTBP1 Antibody [NB100-1310] - (2ug/ml) Human Kidney. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.