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- Western blot [1]
- Immunoprecipitation [1]
- Immunohistochemistry [2]
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- Product number
- 20984-1-AP - Provider product page
- Provider
- Proteintech Group
- Proper citation
- Proteintech Cat#20984-1-AP, RRID:AB_11182390
- Product name
- Raptor antibody
- Antibody type
- Polyclonal
- Description
- KD/KO validated Raptor antibody (Cat. #20984-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse and has been validated for the following applications: IF, IHC, IP, WB, ELISA.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 20ul, 150ul
Submitted references The Deubiquitinase USP39 Promotes ESCC Tumorigenesis Through Pre-mRNA Splicing of the mTORC2 Component Rictor.
CARS senses cysteine deprivation to activate AMPK for cell survival.
Exposure to DEHP induces testis toxicity and injury through the ROS/mTOR/NLRP3 signaling pathway in immature rats.
mTOR Promotes Tissue Factor Expression and Activity in EGFR-Mutant Cancer.
A glycolytic shift in Schwann cells supports injured axons.
Location-specific inhibition of Akt reveals regulation of mTORC1 activity in the nucleus.
Uptake of collagen type I via macropinocytosis cause mTOR activation and anti-cancer drug resistance.
TXNIP deficiency mitigates podocyte apoptosis via restraining the activation of mTOR or p38 MAPK signaling in diabetic nephropathy.
Acetyl-CoA Derived from Hepatic Peroxisomal β-Oxidation Inhibits Autophagy and Promotes Steatosis via mTORC1 Activation.
Quantitative proteomics reveal the alterations in the spinal cord after myocardial ischemia‑reperfusion injury in rats.
The HGF-MET axis coordinates liver cancer metabolism and autophagy for chemotherapeutic resistance.
NOX1-Dependent mTORC1 Activation via S100A9 Oxidation in Cancer Stem-like Cells Leads to Colon Cancer Progression.
mTOR-S6K1 pathway mediates cytoophidium assembly.
The Functional Proximal Proteome of Oncogenic Ras Includes mTORC2.
Thioredoxin-interacting protein deficiency alleviates phenotypic alterations of podocytes via inhibition of mTOR activation in diabetic nephropathy.
UNBS5162 inhibits proliferation of human melanoma cells by inducing apoptosis via the PI3K/Akt pathway.
Ribosomal protein S6 kinase1 coordinates with TOR-Raptor2 to regulate thylakoid membrane biosynthesis in rice.
Arctigenin functions as a selective agonist of estrogen receptor β to restrict mTORC1 activation and consequent Th17 differentiation.
Endostatin Prevents Dietary-Induced Obesity by Inhibiting Adipogenesis and Angiogenesis.
Arctigenin exerts anti-colitis efficacy through inhibiting the differentiation of Th1 and Th17 cells via an mTORC1-dependent pathway.
Ghrelin promotes hepatic lipogenesis by activation of mTOR-PPARγ signaling pathway.
Zhao Y, Geng H, Liu G, Ji Q, Cheng X, Li X, Liu W, Thorne RF, Zhang R, Liu X
Frontiers in oncology 2021;11:667495
Frontiers in oncology 2021;11:667495
CARS senses cysteine deprivation to activate AMPK for cell survival.
Yuan M, Yan R, Zhang Y, Qiu Y, Jiang Z, Liu H, Wang Y, Sun L, Zhang H, Gao P
The EMBO journal 2021 Nov 2;40(21):e108028
The EMBO journal 2021 Nov 2;40(21):e108028
Exposure to DEHP induces testis toxicity and injury through the ROS/mTOR/NLRP3 signaling pathway in immature rats.
Hong Y, Zhou Y, Shen L, Wei Y, Long C, Fu Y, Wu H, Wang J, Wu Y, Wu S, Wei G
Ecotoxicology and environmental safety 2021 Dec 20;227:112889
Ecotoxicology and environmental safety 2021 Dec 20;227:112889
mTOR Promotes Tissue Factor Expression and Activity in EGFR-Mutant Cancer.
Cong Y, Li Q, Zhang X, Chen Y, Yu K
Frontiers in oncology 2020;10:1615
Frontiers in oncology 2020;10:1615
A glycolytic shift in Schwann cells supports injured axons.
Babetto E, Wong KM, Beirowski B
Nature neuroscience 2020 Oct;23(10):1215-1228
Nature neuroscience 2020 Oct;23(10):1215-1228
Location-specific inhibition of Akt reveals regulation of mTORC1 activity in the nucleus.
Zhou X, Zhong Y, Molinar-Inglis O, Kunkel MT, Chen M, Sun T, Zhang J, Shyy JY, Trejo J, Newton AC, Zhang J
Nature communications 2020 Nov 30;11(1):6088
Nature communications 2020 Nov 30;11(1):6088
Uptake of collagen type I via macropinocytosis cause mTOR activation and anti-cancer drug resistance.
Yamazaki S, Su Y, Maruyama A, Makinoshima H, Suzuki J, Tsuboi M, Goto K, Ochiai A, Ishii G
Biochemical and biophysical research communications 2020 May 21;526(1):191-198
Biochemical and biophysical research communications 2020 May 21;526(1):191-198
TXNIP deficiency mitigates podocyte apoptosis via restraining the activation of mTOR or p38 MAPK signaling in diabetic nephropathy.
Song S, Qiu D, Wang Y, Wei J, Wu H, Wu M, Wang S, Zhou X, Shi Y, Duan H
Experimental cell research 2020 Mar 15;388(2):111862
Experimental cell research 2020 Mar 15;388(2):111862
Acetyl-CoA Derived from Hepatic Peroxisomal β-Oxidation Inhibits Autophagy and Promotes Steatosis via mTORC1 Activation.
He A, Chen X, Tan M, Chen Y, Lu D, Zhang X, Dean JM, Razani B, Lodhi IJ
Molecular cell 2020 Jul 2;79(1):30-42.e4
Molecular cell 2020 Jul 2;79(1):30-42.e4
Quantitative proteomics reveal the alterations in the spinal cord after myocardial ischemia‑reperfusion injury in rats.
Li SY, Li ZX, He ZG, Wang Q, Li YJ, Yang Q, Wu DZ, Zeng HL, Xiang HB
International journal of molecular medicine 2019 Nov;44(5):1877-1887
International journal of molecular medicine 2019 Nov;44(5):1877-1887
The HGF-MET axis coordinates liver cancer metabolism and autophagy for chemotherapeutic resistance.
Huang X, Gan G, Wang X, Xu T, Xie W
Autophagy 2019 Jul;15(7):1258-1279
Autophagy 2019 Jul;15(7):1258-1279
NOX1-Dependent mTORC1 Activation via S100A9 Oxidation in Cancer Stem-like Cells Leads to Colon Cancer Progression.
Ohata H, Shiokawa D, Obata Y, Sato A, Sakai H, Fukami M, Hara W, Taniguchi H, Ono M, Nakagama H, Okamoto K
Cell reports 2019 Jul 30;28(5):1282-1295.e8
Cell reports 2019 Jul 30;28(5):1282-1295.e8
mTOR-S6K1 pathway mediates cytoophidium assembly.
Sun Z, Liu JL
Journal of genetics and genomics = Yi chuan xue bao 2019 Feb;46(2):65-74
Journal of genetics and genomics = Yi chuan xue bao 2019 Feb;46(2):65-74
The Functional Proximal Proteome of Oncogenic Ras Includes mTORC2.
Kovalski JR, Bhaduri A, Zehnder AM, Neela PH, Che Y, Wozniak GG, Khavari PA
Molecular cell 2019 Feb 21;73(4):830-844.e12
Molecular cell 2019 Feb 21;73(4):830-844.e12
Thioredoxin-interacting protein deficiency alleviates phenotypic alterations of podocytes via inhibition of mTOR activation in diabetic nephropathy.
Song S, Qiu D, Shi Y, Wang S, Zhou X, Chen N, Wei J, Wu M, Wu H, Duan H
Journal of cellular physiology 2019 Feb 11;
Journal of cellular physiology 2019 Feb 11;
UNBS5162 inhibits proliferation of human melanoma cells by inducing apoptosis via the PI3K/Akt pathway.
Shi X, Yang L, Xie J, Zhao Y, Cong J, Li Z, Li H, Cheng X, Fan J
Molecular medicine reports 2018 Sep;18(3):3382-3388
Molecular medicine reports 2018 Sep;18(3):3382-3388
Ribosomal protein S6 kinase1 coordinates with TOR-Raptor2 to regulate thylakoid membrane biosynthesis in rice.
Sun L, Yu Y, Hu W, Min Q, Kang H, Li Y, Hong Y, Wang X, Hong Y
Biochimica et biophysica acta 2016 Jul;1861(7):639-49
Biochimica et biophysica acta 2016 Jul;1861(7):639-49
Arctigenin functions as a selective agonist of estrogen receptor β to restrict mTORC1 activation and consequent Th17 differentiation.
Wu X, Tong B, Yang Y, Luo J, Yuan X, Wei Z, Yue M, Xia Y, Dai Y
Oncotarget 2016 Dec 20;7(51):83893-83906
Oncotarget 2016 Dec 20;7(51):83893-83906
Endostatin Prevents Dietary-Induced Obesity by Inhibiting Adipogenesis and Angiogenesis.
Wang H, Chen Y, Lu XA, Liu G, Fu Y, Luo Y
Diabetes 2015 Jul;64(7):2442-56
Diabetes 2015 Jul;64(7):2442-56
Arctigenin exerts anti-colitis efficacy through inhibiting the differentiation of Th1 and Th17 cells via an mTORC1-dependent pathway.
Wu X, Dou Y, Yang Y, Bian D, Luo J, Tong B, Xia Y, Dai Y
Biochemical pharmacology 2015 Aug 15;96(4):323-36
Biochemical pharmacology 2015 Aug 15;96(4):323-36
Ghrelin promotes hepatic lipogenesis by activation of mTOR-PPARγ signaling pathway.
Li Z, Xu G, Qin Y, Zhang C, Tang H, Yin Y, Xiang X, Li Y, Zhao J, Mulholland M, Zhang W
Proceedings of the National Academy of Sciences of the United States of America 2014 Sep 9;111(36):13163-8
Proceedings of the National Academy of Sciences of the United States of America 2014 Sep 9;111(36):13163-8
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Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- HepG2 cells were subjected to SDS PAGE followed by western blot with 20984-1-AP(RPTOR antibody) at dilution of 1:1000
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- IP Result of anti-Raptor (IP:20984-1-AP, 4ug; Detection:20984-1-AP 1:1000) with HeLa cells lysate 1505ug.
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemical of paraffin-embedded human kidney using 20984-1-AP(RPTOR antibody) at dilution of 1:50 (under 10x lens)
- Sample type
- tissue
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- The Raptor antibody from Proteintech is a rabbit polyclonal antibody to a peptide of human Raptor. This antibody recognizes human,mouse,rat antigen. The Raptor antibody has been validated for the following applications: ELISA, WB, IHC, IP analysis.
