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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- PAB11258 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB11258, RRID:AB_1713121
- Product name
- ROBO1 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of ROBO1.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Induction of tumor angiogenesis by Slit-Robo signaling and inhibition of cancer growth by blocking Robo activity.
Quantification of expression of netrins, slits and their receptors in human prostate tumors.
Robo1 and Robo2 are homophilic binding molecules that promote axonal growth.
Wang B, Xiao Y, Ding BB, Zhang N, Yuan Xb, Gui L, Qian KX, Duan S, Chen Z, Rao Y, Geng JG
Cancer cell 2003 Jul;4(1):19-29
Cancer cell 2003 Jul;4(1):19-29
Quantification of expression of netrins, slits and their receptors in human prostate tumors.
Latil A, Chêne L, Cochant-Priollet B, Mangin P, Fournier G, Berthon P, Cussenot O
International journal of cancer 2003 Jan 20;103(3):306-15
International journal of cancer 2003 Jan 20;103(3):306-15
Robo1 and Robo2 are homophilic binding molecules that promote axonal growth.
Hivert B, Liu Z, Chuang CY, Doherty P, Sundaresan V
Molecular and cellular neurosciences 2002 Dec;21(4):534-45
Molecular and cellular neurosciences 2002 Dec;21(4):534-45
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot using ROBO1 polyclonal antibody (Cat # PAB11258) shows detection of a band at ~181 kDa correspond-ing to ROBO1 present in mouse brain lysate (arrowhead).Approximately 35 ug of lysate was separated by 4-8% SDS-PAGE and probed with the primary antibody diluted to 1:1,000.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunohistochemistry of ROBO1 polyclonal antibody (Cat # PAB11258) was used at a concentration of 5 ug/mL to detect ROBO1 in a variety of tissues including multi-human, multi-brain and multi-cancer slides.This image shows staining of human brain tissue.Tissue was formalin-fixed and paraffin embedded.Personal Communication, Tina Roush, Life Span Biosciences, Seattle, WA.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
