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- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunohistochemistry [2]
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- Product number
- NB600-1254 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB600-1254, RRID:AB_2181694
- Product name
- Rabbit Polyclonal ROBO1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. We have tested this by Western blotting on human lung and brain and have seen no band corresponding to the expected size for robo1, however we are aware that expression of this protein is very low in most tissues. Expected to run at 180kD.
- Reactivity
- Human, Mouse, Rat, Canine
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1.0 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ROBO1 Antibody [NB600-1254] - Detection of a band at ~181 kDa corresponding to ROBO-1 present in mouse brain lysate (arrowhead). Approximately 35 ug of lysate was separated by 4-8% SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:1,000. Reaction occurred 2h at room temperature followed by washes and reaction with a 1:10,000 dilution of IRDye (TM) 800 conjugated Gt-a-Rabbit IgG [H&L] MX for 45 min at room temperature.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ROBO1 Antibody [NB600-1254] - ylated human mTOR present in a 293T whole cell lysates. Cells were serum-starved for 24 hours prior to harvest. ~20ug of lysate was loaded per lane for SDS-PAGE. Untreated cells are shown in lane 1, whereas cells in lane 2 were treated with IGF-1 (100 ng/ml) for 20 min prior to harvest. Follow reaction of antibody with a 1:2000 dilution of HRP Goat-a-Rabbit IgG for visualization.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ROBO1 Antibody [NB600-1254] - Lane 1: HeLa WCL. Load: 30 ug per lane. Primary antibody: Robo-1 antibody at 1:1,000 for overnight at 4C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:40,000 for 30 min at RT. Block: Blocking Buffer for Fluorescent Western Blotting for 30 min at RT. Predicted/Observed size: 181 kDa, 181 kDa for Robo-1. Other band(s): not identified.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Frozen: ROBO1 Antibody [NB600-1254] - 1/50 staining mouse lung tissue sections (adult, frozen 100um wholemount sections) by IHC-Fr. The tissue was paraformaldehyde fixed and permeabilized with triton x-100 before incubation with the antibody for 16 hours at 4C.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: ROBO1 Antibody [NB600-1254] - Used at a concentration of 5 ug/ml to detect ROBO1 in a variety of tissues including multi-human, multi-brain and multi-cancer slides. This image shows staining of human brain tissue. Tissue was formalin-fixed and paraffin embedded.
