AP09258PU-N

antibody from Acris Antibodies GmbH

Targeting: ROBO1 DUTT1, FLJ21882, SAX3

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AP09258PU-N

Provider

Acris Antibodies GmbH

Proper citation

Acris Antibodies GmbH Cat#AP09258PU-N, RRID:AB_2035924

Product name

anti ROBO1 (1632-1644)

Antibody type

Polyclonal

Antigen

Synthetic peptide corresponding to amino acids 1632-1644 of Human ROBO-1

Reactivity

Human, Mouse, Rat, Canine

Host

Rabbit

Isotype

IgG

Vial size

0.1 mg

Concentration

1.0 mg/ml (by UV absorbance at 280 nm)

Western blot

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Western blot using AP09258PU-N ROBO-1 antibody shows detection of a band at ~181 kDa corresponding to ROBO-1 present in Mouse brain lysate (arrowhead). Approximately 35 μg of lysate was separated by 4-8% SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:1,000. Reaction occurred 2h at room temperature followed by washes and reaction with a 1:10,000 dilution of IRDye(TM)800 conjugated Goat anti-Rabbit IgG [H&L] for 45 min at RT. IRDye(TM)800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is trademark of LI-COR, Inc. Other detection systems will yield similar results.

Immunohistochemistry

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Immunohistochemistry: AP09258PU-N ROBO1 antibody was used at a concentration of 5 μg/ml to detect ROBO1 in a variety of tissues including multi-human, multi-brain and multi-cancer slides. This image shows staining of Human brain tissue. Tissue was Formalin-Fixed, Paraffin Eembedded. Personal Communication, Tina Roush, LifeSpanBiosciences, Seattle, WA.