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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [2]
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- Product number
- GTX36928 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX36928, RRID:AB_11178761
- Product name
- Robo1 antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat, Canine
- Host
- Rabbit
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Western blot using Affinity Purified anti-ROBO-1 antibody (GTX36928) shows detection of a band at ~181 kDa corresponding to ROBO-1 present in mouse brain lysate (arrowhead). Approximately 35 ?g of lysate was separated by 4-8% SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:1,000. Reaction occurred 2h at room temperature followed by washes and reaction with a 1:10,000 dilution of infrared 800 conjugated Gt-a-Rabbit IgG [H&L] MX for 45 min at room temperature. Infrared 800 fluorescence image was captured using the Odyssey? Infrared Imaging System developed by LI-COR.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Affinity Purified anti-ROBO1 antibody (GTX36928) was used at a concentration of 5 ?g/ml to detect ROBO1 in a variety of tissues including multi-human, multi-brain and multi-cancer slides. This image shows staining of human brain tissue. Tissue was formalin-fixed and paraffin embedded.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- 1/50 staining mouse lung tissue sections (adult, frozen 100?m wholemount sections) by IHC-Fr. The tissue was paraformaldehyde fixed and permeabilized with polyethylene glycol tert-octylphenyl ether before incubation with the antibody (GTX36928) for 16 hours at 4¢XC.
