16993-1-AP

antibody from Invitrogen Antibodies

Targeting: MGAT5B FLJ25132, GnT-IX, GnT-VB

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

16993-1-AP

Provider

Invitrogen Antibodies

Product name

MGAT5B Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: MITVNPDGK IMVRRCLVTL RPFRLFVLGI GFFTLCFLMT SLGGQFSARR LGDSPFTIRT EVMGGPESRG VLRKMSDLLE LMVKRMDALA RLENSSELHR AGGDLHFPAD RMPPGAGLME RIQAIAQNVS DIAVKVDQIL RHSLLLHSKV SEGRRDQCEA PSDPKFPDCS GKVEWMRARW TSDPCYAFFG VDGTECSFLI YLSEVEWFCP PLPWRNQTAA QRAPKPLPKV QAVFRSNLSH LLDLMGSGKE SLIFMKKRTK RLTAQWALAA QRLAQKLGAT QRDQKQILVH IGFLTEESGD VFSPRVLKGG PLGEMVQWAD ILTALYVLGH GLRVTVSLKE LQRQRRLRSS QEQARCGVMG ACEKMLLEGD VASNTLLIEK PSKKETEKCP KNLVT (1-394 aa encoded by BC062354)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.28 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 16993-1-AP (MGAT5B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HepG2 cells were subjected to SDS PAGE followed by western blot with 16993-1-AP (MGAT5B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

C6 cells were subjected to SDS PAGE followed by western blot with 16993-1-AP (MGAT5B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Jurkat cells were subjected to SDS PAGE followed by western blot with 16993-1-AP (MGAT5B antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Jurkat cells were subjected to SDS PAGE followed by western blot with 16993-1-AP (MGAT5B antibody) at dilution of 1:1600 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of ( -20°C Ethanol ) fixed HepG2 cells using 16993-1-AP (MGAT5B antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded mouse brain tissue slide using 16993-1-AP (MGAT5B antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded mouse brain tissue slide using 16993-1-AP (MGAT5B antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded mouse brain tissue slide using 16993-1-AP (MGAT5B antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded mouse brain tissue slide using 16993-1-AP (MGAT5B antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-MGAT5B (IP:16993-1-AP, 4ug; Detection:16993-1-AP 1:700) with Jurkat cells lysate 3600ug.