GTX81821

antibody from GeneTex

Targeting: KLF6 BCD1, COPEB, CPBP, GBF, PAC1, ST12, Zf9

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

GTX81821

Provider

GeneTex

Proper citation

GeneTex Cat#GTX81821, RRID:AB_11166634

Product name

KLF6 antibody, C-term

Antibody type

Polyclonal

Reactivity

Human, Mouse

Host

Rabbit

Storage

Keep as concentrated solution. For short-term storage, store at 4¢X C (up to 10 days). For long-term storage, aliquot and store at -20?C or below. Avoid multiple freeze-thaw cycles.

References

Submitted references

Meningeal SWI/SNF related, matrix-associated, actin-dependent regulator of chromatin, subfamily B member 1 (SMARCB1)-deficient tumours: an emerging group of meningeal tumours.

Dadone B, Fontaine D, Mondot L, Cristofari G, Jouvet A, Godfraind C, Varlet P, Ranchère-Vince D, Coindre JM, Gastaud L, Baudoin C, Peyron AC, Thyss A, Coutts M, Michiels JF, Pedeutour F, Burel-Vandenbos F, RENOP.
Neuropathology and applied neurobiology 2017 Aug;43(5):433-449

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Krüppel-like factor 5 as potential molecular marker in cervical cancer and the KLF family profile expression.

Marrero-Rodríguez D, Taniguchi-Ponciano K, Jimenez-Vega F, Romero-Morelos P, Mendoza-Rodríguez M, Mantilla A, Rodriguez-Esquivel M, Hernandez D, Hernandez A, Gomez-Gutierrez G, Muñoz-Hernandez N, la Cruz HA, Vargas-Requena C, Díaz-Hernández C, Serna-Reyna L, Meraz-Rios M, Bandala C, Ortiz-Leon J, Salcedo M
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine 2014 Nov;35(11):11399-407

Western blot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Western blot analysis of KLF6 Antibody (C-term) (GTX81821) in mouse stomach tissue lysates (35ug/lane). KLF6 (arrow) was detected using the purified Pab.

Validation comment

WB

Immunocytochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Fluorescent confocal image of Hela cell stained with KLF6 Antibody (C-term)(GTX81821).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with KLF6 primary antibody (1:25, 1 h at 37¢J). For secondary antibody, Alexa Fluor? 488 conjμgated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37¢J).Cytoplasmic actin was counterstained with Alexa Fluor? 555 (red) conjμgated Phalloidin (7units/ml, 1 h at 37¢J). Nuclei were counterstained with DAPI (blue) (10 μg/ml, 10 min). KLF6 immunoreactivity is localized to Nucleus significantly.

Immunohistochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Formalin-fixed and paraffin-embedded human testis tissue reacted with KLF6 Antibody (C-term)(GTX81821), which was peroxidase-conjμgated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Flow cytometry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Flow cytometric analysis of widr cells using KLF6 Antibody (C-term)(bottom histogram)(GTX81821)compared to a negative control cell (top histogram)FITC-conjμgated goat-anti-rabbit secondary antibodies were used for the analysis.