- PA5-79560 - Invitrogen Antibodies KLF6 antibody

Kiel C, Strunz T, Hasler D, Meister G, Grassmann F, Weber BHF
Human molecular genetics 2022 Mar 21;31(6):875-887

Sun J, Cai J, Chen J, Li S, Liao X, He Y, Chen X, Hu S
Frontiers in aging neuroscience 2021;13:646729

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of KLF6 in Lane 1: human placenta tissue lysate, Lane 2: rat testis tissue lysate, Lane 3: HeLa whole cell lysate, Lane 4: HEPG2 whole cell lysate, Lane 5: HEPA whole cell lysate using 40-50 µg per well. Sample was incubated with KLF6 (Product # PA5-79560) at a dilution of 0.5 µg/mL.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of KLF6 in recombinant human KLF6 protein using 0.5 ng per well. Sample was incubated with KLF6 polyclonal antibody (Product # PA5-79560) at a dilution of 0.5 µg/mL.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 1 The expression of KLF6 is high in hippocampal neurons of ICH model rats. (A) The expression of KLF6 in the ICH-related microarray GSE149317. The box diagram shows the expression of KLF6 gene; the red one indicates the ICH group, and the gray one indicates the normal group. (B) KLF6 expression in rats at different time points (6, 12, 24, 72 h, 7 days) after ICH modeling determined by Western blot analysis. (C) KLF6 expression and location in different neurons detected by immunofluorescence staining. * p < 0.05 compared with sham-operated rats. The measurement data were expressed as mean +- standard deviation. Comparisons between two groups were analyzed using unpaired t -test, and comparisons among multiple groups were tested by one-way ANOVA followed by Dunnett post-hoc test. n = 6 rats/group.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 5 KLF6 silencing depresses OxyHb-induced mitochondrial ROS and mitochondrial damage in hippocampal neurons. (A) The level of KLF6 in hippocampal neurons after KLF6 silencing determined by RT-qPCR. (B) The level of KLF6 in hippocampal neurons after KLF6 silencing determined by Western blot analysis. (C) The expression of KLF6 in hippocampal neurons after 20 muM OxyHb induction and KLF6 silencing determined by Western blot analysis. (D) The effect of KLF6 silencing on OxyHb-induced hippocampal neuronal apoptosis determined by flow cytometry. (E) The changes of mitochondrial ROS in neurons after 20 muM OxyHb induction and KLF6 silencing detected by MitoSOX fluorescence staining. (F) Mitochondrial membrane potential changes after 20 muM OxyHb induction and KLF6 silencing determined by JC-1 staining. * p < 0.05. The measurement data were expressed as mean +- standard deviation. Comparisons between two groups were analyzed using unpaired t -test, and comparisons among multiple groups were tested by one-way ANOVA followed by Dunnett post-hoc test. The experiment was repeated three times.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Allele-specific hsa-miR-4513 target gene protein expression. Representative western blot analyses of five selected target genes with the respective loading controls ACTB or HSP90 (for KLF6). Isoform p16 represents one of the protein products of CDKN2A . All samples were normalized to their respective loading control ACTB or HSP90 and the control miRNA cel-miR-39. Shown are mean values from 9 to 17 independent experiments. Bars indicate SE. Statistical significance was determined with the Kruskal-Wallis test followed by a Dunn's multiple comparison test to adjust for multiple testing. * Adjusted P -value < 0.05.

PA5-79560