PA5-92110

antibody from Invitrogen Antibodies

Targeting: NDUFB2 AGGG, CI-AGGG

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-92110

Provider

Invitrogen Antibodies

Product name

NDUFB2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: AGGGVHIEPR YRQFPQLTRS QVFQSEFFSG LMWFWILWRF WHDSEEVLGH FPYPDPSQWT DEELGIPPDD ED; Positive Samples: NCI-H460, HeLa, 293T, Mouse brain, Mouse heart, Mouse kidney, Mouse liver; Cellular Location: Matrix side, Mitochondrion inner membrane, Peripheral membrane protein

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.82 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of extracts of various cell lines, using NDUFB2 Polyclonal antibody (Product # PA5-92110) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Exposure time: 30s.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of NDUFB2 in extracts of various cell lines using NDUFB2 Polyclonal Antibody (Product # PA5-92110) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of NDUFB2 was performed in L929 cells using NDUFB2 Polyclonal Antibody (Product # PA5-92110) at a dilution of 1:100. Blue: DAPI for nuclear staining.