A305-823A-T

antibody from Invitrogen Antibodies

Targeting: SPCS1 HSPC033, SPC1, SPC12, YJR010C-A

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

A305-823A-T

Provider

Invitrogen Antibodies

Product name

SPCS1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

10 µL

Concentration

1 mg/mL

Storage

4° C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human SPCS1 by western blot. Samples: Whole cell lysate (15 µg) from HeLa, Jurkat, and MCF-7 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SPCS1 antibody A305-823A-M (lot A305-823A-M-1) used for WB at 1:1000. Detection: Chemiluminescence with an exposure time of 10 seconds.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human SPCS1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-SPCS1 antibody (Product # A305-823A-T; lot A305-823A-T-1) used for IP at 100 µL per reaction. SPCS1 was also immunoprecipitated by rabbit anti-SPCS1 antibody BL23631. For blotting immunoprecipitated SPCS1, Product # A305-823A-T was used at 1:1000. Detection: Chemiluminescence with an exposure time of 10 seconds.