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- Antigen structure
- References [28]
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- Immunohistochemistry [1]
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- Product number
- AF446 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal EphB4 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects mouse EphB4 in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant mouse (rm) EphB6 and rmEphA3 is observed and less than 1% cross-reactivity with rmEphA2, rmEphA4, rmEphA6, rmEphA7, rmEphA8, rmEphB2, and rmEphB3 is observed.
- Reactivity
- Mouse
- Host
- Goat
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Endothelial EphB4 maintains vascular integrity and transport function in adult heart.
Regulatory pathways governing murine coronary vessel formation are dysregulated in the injured adult heart.
Venous identity requires BMP signalling through ALK3.
EphrinB2/EphB4 signaling regulates non-sprouting angiogenesis by VEGF.
Histochemical assessment for osteoblastic activity coupled with dysfunctional osteoclasts in c-src deficient mice.
Eph-B4 mediates vein graft adaptation by regulation of endothelial nitric oxide synthase.
EphrinB2 repression through ZEB2 mediates tumour invasion and anti-angiogenic resistance.
Downregulation of proinflammatory cytokines in HTLV-1-infected T cells by Resveratrol.
EPHB4 kinase-inactivating mutations cause autosomal dominant lymphatic-related hydrops fetalis.
EphB4 Expressing Stromal Cells Exhibit an Enhanced Capacity for Hematopoietic Stem Cell Maintenance.
The in vivo effect of prophylactic subchondral bone protection of osteoarthritic synovial membrane in bone-specific Ephb4-overexpressing mice.
EphB4 forward signalling regulates lymphatic valve development.
Molecular identification of venous progenitors in the dorsal aorta reveals an aortic origin for the cardinal vein in mammals.
Sox17 is required for normal pulmonary vascular morphogenesis.
Single-cell western blotting.
A novel feedback mechanism by Ephrin-B1/B2 in T-cell activation involves a concentration-dependent switch from costimulation to inhibition.
Eph-B4 prevents venous adaptive remodeling in the adult arterial environment.
Direct transcriptional regulation of neuropilin-2 by COUP-TFII modulates multiple steps in murine lymphatic vessel development.
Ephrin-B2 controls VEGF-induced angiogenesis and lymphangiogenesis.
EphB-ephrin-B2 interactions are required for thymus migration during organogenesis.
Endothelial-specific expression of WNK1 kinase is essential for angiogenesis and heart development in mice.
Dynamic changes occur in patterns of endometrial EFNB2/EPHB4 expression during the period of spiral arterial modification in mice.
Artery and vein size is balanced by Notch and ephrin B2/EphB4 during angiogenesis.
EphrinB2 regulation by PTH and PTHrP revealed by molecular profiling in differentiating osteoblasts.
The EphB4 receptor-tyrosine kinase promotes the migration of melanoma cells through Rho-mediated actin cytoskeleton reorganization.
EphB2 and ephrin-B1 expressed in the adult kidney regulate the cytoarchitecture of medullary tubule cells through Rho family GTPases.
Inhibition of tumor growth and angiogenesis by soluble EphB4.
Forward EphB4 signaling in endothelial cells controls cellular repulsion and segregation from ephrinB2 positive cells.
Luxán G, Stewen J, Díaz N, Kato K, Maney SK, Aravamudhan A, Berkenfeld F, Nagelmann N, Drexler HC, Zeuschner D, Faber C, Schillers H, Hermann S, Wiseman J, Vaquerizas JM, Pitulescu ME, Adams RH
eLife 2019 Nov 29;8
eLife 2019 Nov 29;8
Regulatory pathways governing murine coronary vessel formation are dysregulated in the injured adult heart.
Payne S, Gunadasa-Rohling M, Neal A, Redpath AN, Patel J, Chouliaras KM, Ratnayaka I, Smart N, De Val S
Nature communications 2019 Jul 22;10(1):3276
Nature communications 2019 Jul 22;10(1):3276
Venous identity requires BMP signalling through ALK3.
Neal A, Nornes S, Payne S, Wallace MD, Fritzsche M, Louphrasitthiphol P, Wilkinson RN, Chouliaras KM, Liu K, Plant K, Sholapurkar R, Ratnayaka I, Herzog W, Bond G, Chico T, Bou-Gharios G, De Val S
Nature communications 2019 Jan 28;10(1):453
Nature communications 2019 Jan 28;10(1):453
EphrinB2/EphB4 signaling regulates non-sprouting angiogenesis by VEGF.
Groppa E, Brkic S, Uccelli A, Wirth G, Korpisalo-Pirinen P, Filippova M, Dasen B, Sacchi V, Muraro MG, Trani M, Reginato S, Gianni-Barrera R, Ylä-Herttuala S, Banfi A
EMBO reports 2018 May;19(5)
EMBO reports 2018 May;19(5)
Histochemical assessment for osteoblastic activity coupled with dysfunctional osteoclasts in c-src deficient mice.
Toray H, Hasegawa T, Sakagami N, Tsuchiya E, Kudo A, Zhao S, Moritani Y, Abe M, Yoshida T, Yamamoto T, Yamamoto T, Oda K, Udagawa N, Luiz de Freitas PH, Li M
Biomedical research (Tokyo, Japan) 2017;38(2):123-134
Biomedical research (Tokyo, Japan) 2017;38(2):123-134
Eph-B4 mediates vein graft adaptation by regulation of endothelial nitric oxide synthase.
Wang M, Collins MJ, Foster TR, Bai H, Hashimoto T, Santana JM, Shu C, Dardik A
Journal of vascular surgery 2017 Jan;65(1):179-189
Journal of vascular surgery 2017 Jan;65(1):179-189
EphrinB2 repression through ZEB2 mediates tumour invasion and anti-angiogenic resistance.
Depner C, Zum Buttel H, Böğürcü N, Cuesta AM, Aburto MR, Seidel S, Finkelmeier F, Foss F, Hofmann J, Kaulich K, Barbus S, Segarra M, Reifenberger G, Garvalov BK, Acker T, Acker-Palmer A
Nature communications 2016 Jul 29;7:12329
Nature communications 2016 Jul 29;7:12329
Downregulation of proinflammatory cytokines in HTLV-1-infected T cells by Resveratrol.
Fuggetta MP, Bordignon V, Cottarelli A, Macchi B, Frezza C, Cordiali-Fei P, Ensoli F, Ciafrè S, Marino-Merlo F, Mastino A, Ravagnan G
Journal of experimental & clinical cancer research : CR 2016 Jul 22;35(1):118
Journal of experimental & clinical cancer research : CR 2016 Jul 22;35(1):118
EPHB4 kinase-inactivating mutations cause autosomal dominant lymphatic-related hydrops fetalis.
Martin-Almedina S, Martinez-Corral I, Holdhus R, Vicente A, Fotiou E, Lin S, Petersen K, Simpson MA, Hoischen A, Gilissen C, Jeffery H, Atton G, Karapouliou C, Brice G, Gordon K, Wiseman JW, Wedin M, Rockson SG, Jeffery S, Mortimer PS, Snyder MP, Berland S, Mansour S, Makinen T, Ostergaard P
The Journal of clinical investigation 2016 Aug 1;126(8):3080-8
The Journal of clinical investigation 2016 Aug 1;126(8):3080-8
EphB4 Expressing Stromal Cells Exhibit an Enhanced Capacity for Hematopoietic Stem Cell Maintenance.
Nguyen TM, Arthur A, Panagopoulos R, Paton S, Hayball JD, Zannettino AC, Purton LE, Matsuo K, Gronthos S
Stem cells (Dayton, Ohio) 2015 Sep;33(9):2838-49
Stem cells (Dayton, Ohio) 2015 Sep;33(9):2838-49
The in vivo effect of prophylactic subchondral bone protection of osteoarthritic synovial membrane in bone-specific Ephb4-overexpressing mice.
Valverde-Franco G, Hum D, Matsuo K, Lussier B, Pelletier JP, Fahmi H, Kapoor M, Martel-Pelletier J
The American journal of pathology 2015 Feb;185(2):335-46
The American journal of pathology 2015 Feb;185(2):335-46
EphB4 forward signalling regulates lymphatic valve development.
Zhang G, Brady J, Liang WC, Wu Y, Henkemeyer M, Yan M
Nature communications 2015 Apr 13;6:6625
Nature communications 2015 Apr 13;6:6625
Molecular identification of venous progenitors in the dorsal aorta reveals an aortic origin for the cardinal vein in mammals.
Lindskog H, Kim YH, Jelin EB, Kong Y, Guevara-Gallardo S, Kim TN, Wang RA
Development (Cambridge, England) 2014 Mar;141(5):1120-8
Development (Cambridge, England) 2014 Mar;141(5):1120-8
Sox17 is required for normal pulmonary vascular morphogenesis.
Lange AW, Haitchi HM, LeCras TD, Sridharan A, Xu Y, Wert SE, James J, Udell N, Thurner PJ, Whitsett JA
Developmental biology 2014 Mar 1;387(1):109-20
Developmental biology 2014 Mar 1;387(1):109-20
Single-cell western blotting.
Hughes AJ, Spelke DP, Xu Z, Kang CC, Schaffer DV, Herr AE
Nature methods 2014 Jul;11(7):749-55
Nature methods 2014 Jul;11(7):749-55
A novel feedback mechanism by Ephrin-B1/B2 in T-cell activation involves a concentration-dependent switch from costimulation to inhibition.
Kawano H, Katayama Y, Minagawa K, Shimoyama M, Henkemeyer M, Matsui T
European journal of immunology 2012 Jun;42(6):1562-72
European journal of immunology 2012 Jun;42(6):1562-72
Eph-B4 prevents venous adaptive remodeling in the adult arterial environment.
Muto A, Yi T, Harrison KD, Dávalos A, Fancher TT, Ziegler KR, Feigel A, Kondo Y, Nishibe T, Sessa WC, Dardik A
The Journal of experimental medicine 2011 Mar 14;208(3):561-75
The Journal of experimental medicine 2011 Mar 14;208(3):561-75
Direct transcriptional regulation of neuropilin-2 by COUP-TFII modulates multiple steps in murine lymphatic vessel development.
Lin FJ, Chen X, Qin J, Hong YK, Tsai MJ, Tsai SY
The Journal of clinical investigation 2010 May;120(5):1694-707
The Journal of clinical investigation 2010 May;120(5):1694-707
Ephrin-B2 controls VEGF-induced angiogenesis and lymphangiogenesis.
Wang Y, Nakayama M, Pitulescu ME, Schmidt TS, Bochenek ML, Sakakibara A, Adams S, Davy A, Deutsch U, Lüthi U, Barberis A, Benjamin LE, Mäkinen T, Nobes CD, Adams RH
Nature 2010 May 27;465(7297):483-6
Nature 2010 May 27;465(7297):483-6
EphB-ephrin-B2 interactions are required for thymus migration during organogenesis.
Foster KE, Gordon J, Cardenas K, Veiga-Fernandes H, Makinen T, Grigorieva E, Wilkinson DG, Blackburn CC, Richie E, Manley NR, Adams RH, Kioussis D, Coles MC
Proceedings of the National Academy of Sciences of the United States of America 2010 Jul 27;107(30):13414-9
Proceedings of the National Academy of Sciences of the United States of America 2010 Jul 27;107(30):13414-9
Endothelial-specific expression of WNK1 kinase is essential for angiogenesis and heart development in mice.
Xie J, Wu T, Xu K, Huang IK, Cleaver O, Huang CL
The American journal of pathology 2009 Sep;175(3):1315-27
The American journal of pathology 2009 Sep;175(3):1315-27
Dynamic changes occur in patterns of endometrial EFNB2/EPHB4 expression during the period of spiral arterial modification in mice.
Zhang J, Dong H, Wang B, Zhu S, Croy BA
Biology of reproduction 2008 Sep;79(3):450-8
Biology of reproduction 2008 Sep;79(3):450-8
Artery and vein size is balanced by Notch and ephrin B2/EphB4 during angiogenesis.
Kim YH, Hu H, Guevara-Gallardo S, Lam MT, Fong SY, Wang RA
Development (Cambridge, England) 2008 Nov;135(22):3755-64
Development (Cambridge, England) 2008 Nov;135(22):3755-64
EphrinB2 regulation by PTH and PTHrP revealed by molecular profiling in differentiating osteoblasts.
Allan EH, Häusler KD, Wei T, Gooi JH, Quinn JM, Crimeen-Irwin B, Pompolo S, Sims NA, Gillespie MT, Onyia JE, Martin TJ
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research 2008 Aug;23(8):1170-81
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research 2008 Aug;23(8):1170-81
The EphB4 receptor-tyrosine kinase promotes the migration of melanoma cells through Rho-mediated actin cytoskeleton reorganization.
Yang NY, Pasquale EB, Owen LB, Ethell IM
The Journal of biological chemistry 2006 Oct 27;281(43):32574-86
The Journal of biological chemistry 2006 Oct 27;281(43):32574-86
EphB2 and ephrin-B1 expressed in the adult kidney regulate the cytoarchitecture of medullary tubule cells through Rho family GTPases.
Ogawa K, Wada H, Okada N, Harada I, Nakajima T, Pasquale EB, Tsuyama S
Journal of cell science 2006 Feb 1;119(Pt 3):559-70
Journal of cell science 2006 Feb 1;119(Pt 3):559-70
Inhibition of tumor growth and angiogenesis by soluble EphB4.
Martiny-Baron G, Korff T, Schaffner F, Esser N, Eggstein S, Marmé D, Augustin HG
Neoplasia (New York, N.Y.) 2004 May-Jun;6(3):248-57
Neoplasia (New York, N.Y.) 2004 May-Jun;6(3):248-57
Forward EphB4 signaling in endothelial cells controls cellular repulsion and segregation from ephrinB2 positive cells.
Füller T, Korff T, Kilian A, Dandekar G, Augustin HG
Journal of cell science 2003 Jun 15;116(Pt 12):2461-70
Journal of cell science 2003 Jun 15;116(Pt 12):2461-70
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- EphB4 in Mouse Embryo. EphB4 was detected in immersion fixed frozen sections of mouse embryo (15 d.p.c.) using Goat Anti-Mouse EphB4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF446) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
