NB200-194

antibody from Novus Biologicals

Targeting: DDX3X DBX, DDX14, DDX3, HLP2

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

NB200-194

Provider

Novus Biologicals

Proper citation

Novus Cat#NB200-194, RRID:AB_10001348

Product name

Rabbit Polyclonal DDX3 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified.

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

0.1 ml

Concentration

0.2 mg/ml

Storage

Store at 4C. Do not freeze.

References

Submitted references

The DEAD-box RNA helicase DDX3 interacts with DDX5, co-localizes with it in the cytoplasm during the G2/M phase of the cycle, and affects its shuttling during mRNP export.

Choi YJ, Lee SG
Journal of cellular biochemistry 2012 Mar;113(3):985-96

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Human cytomegalovirus induces the interferon response via the DNA sensor ZBP1.

DeFilippis VR, Alvarado D, Sali T, Rothenburg S, Früh K
Journal of virology 2010 Jan;84(1):585-98

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Viral targeting of DEAD box protein 3 reveals its role in TBK1/IKKepsilon-mediated IRF activation.

Schröder M, Baran M, Bowie AG
The EMBO journal 2008 Aug 6;27(15):2147-57

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Differential gene expression profiling in HELLP syndrome placentas.

Kang BY, Tsoi S, Zhu S, Su S, Kay HH
Reproductive sciences (Thousand Oaks, Calif.) 2008 Apr;15(3):285-94

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: DDX3 Antibody [NB200-194] - Detection of DDX3 by Western Blot and Immunoprecipitation. Samples: A. Whole cell lysate from HeLa (5, 15 and 50 mcg) and Ramos (50 mcg) cells. B. Whole cell lysate (1 mg/IP; 1/4 of IP loaded/lane) from HeLa cells. Antibodies: NB 200-194 used at 0.04 mcg/ml (A) and 0.1 mcg/ml (B) for Western Blot and at 3 mcg/mg lysate for IP (B). DDX3 was also immunoprecipitated using NB 200-195 and NB 200-196 at 3 mcg/mg lysate. Detection: Chemiluminescence with an exposure time of 10 seconds (A and B).

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: DDX3 Antibody [NB200-194] - Detection of human and mouse DDX3 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, TCMK-1, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-DDX3 antibody NB200-194 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry: DDX3 Antibody [NB200-194] - Sample: FFPE section of human lung cancer pleural effusion (left) and mouse renal cell carcinoma (right). Antibody: Affinity purified rabbit anti-DDX3 used at a dilution of 1:200 (1ug/ml). Detection: Red fluorescence (A120-601D4) and DAB.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry: DDX3 Antibody [NB200-194] - Detection of mouse DDX3 by immunohistochemistry. Sample: FFPE section of mouse plasmacytoma. Antibody: Affinity purified rabbit anti-DDX3 (NB200-194 lot 2) used at a dilution of 1:200 (1ug/ml). Detection: DAB.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry: DDX3 Antibody [NB200-194] - Detection of human DDX3 by immunohistochemistry. Sample: FFPE section of human lung cancer. Antibody: Affinity purified rabbit anti-DDX3 (NB200-194 lot 2) used at a dilution of 1:200 (1ug/ml). Detection: DAB.