HPA005631

antibody from Atlas Antibodies

Targeting: DDX3X DBX, DDX14, DDX3, HLP2

Western blot (Enhanced validation)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

HPA005631

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#HPA005631, RRID:AB_1078633

Product name

Anti-DDX3X

Antibody type

Polyclonal

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Antigen sequence

LPSDIEEYVHRIGRTGRVGNLGLATSFFNERNINI
TKDLLDLLVEAKQEVPSWLENMAYEHHYKGSSRGR
SKSSRFSGGFGARDYRQSSGASSSSFSSSRASSSR
SGGGGHGSSRGFGGGGYGG


Isotype

IgG

Vial size

100 µl

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Western blot

Supportive validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western blot analysis in Rh30 cells transfected with control siRNA, target specific siRNA probe #1, using Anti-DDX3X antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.

Enhanced validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Independent antibody validation

Main image

Experimental details

Western blot analysis using Anti-DDX3X antibody HPA005631 (A) shows similar pattern to independent antibody HPA001648 (B).

Sample type

HUMAN

Antibody #2 product nr

HPA001648

Antibody provider

Atlas Antibodies

Independent Antibody Method

WB

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Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells)Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells)

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Lane 1: Marker [kDa] 220, 112, 84, 47, 32, 26, 17Lane 2: Human cell line RT-4Lane 3: Human cell line U-251MG sp

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10Lane 2: Mouse Cerebral Cortex tissue

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Western blot analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.

Immunocytochemistry

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.

Sample type

HUMAN

Immunohistochemistry

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human liver shows weak cytoplasmic positivity in hepatocytes.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human lung shows moderate cytoplasmic positivity in macrophages.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human lymphoid tissues shows moderate cytoplasmic positivity in germinal center cells.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human placenta shows moderate cytoplasmic positivity in trophoblastic cells.

Sample type

HUMAN